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Ordering Information
- : 16-184
- : 10 blots
Product Images
Western Blot Analysis:
This lot detected tyrosine-phosphorylated proteins in RIPA lysates from EGF-treated A431 cells tested at 1:5000 dilution i...
Anti-Phosphotyrosine, recombinant clone 4G10®, HRP conjugate
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| A | WB | Mouse | HRP | Monoclonal Antibody |
Description:
Anti-Phosphotyrosine, recombinant clone 4G10®, HRP conjugate
Trade Name:
Upstate (Millipore)
Product Overview:
Anti-phosphotyrosine antibody (05-321) cross-linked to horseradish peroxidase (HRP).
Specificity:
Recognizes tyrosine-phosphorylated proteins from all species.
Molecular Weight:
Varies
Epitope:
N/A
Immunogen:
Phosphotyramine-KLH.
Modifications:
Phosphorylation
Clone:
4G10®
Isotype:
IgG2bκ
Background Information:
The phosphorylation of specific tyrosine residues has been shown to be a primary mechanism of signal transduction during normal mitogenesis, cell cycle progression and oncogenic transformation, its role in other areas such as differentiation and gap junction communication, is a matter of active and ongoing research. Antibodies that specifically recognize phosphorylated tyrosine residues have proved to be invaluable to the study of tyrosine phosphorylated proteins and the biochemical pathways in which they function.
Description
Recombinant 4G10®, produced in Chinese Hamster Ovary (CHO) cells expressing the 4G10® antibody heavy and light chain cDNAs. Heavy chain C-terminus has a hexa-histidine (His6) tag for purification and immobilization via nickel affinity matrices. Cross-linked to horseradish peroxidase (HRP). Patent pending.
Description
Recombinant 4G10®, produced in Chinese Hamster Ovary (CHO) cells expressing the 4G10® antibody heavy and light chain cDNAs. Heavy chain C-terminus has a hexa-histidine (His6) tag for purification and immobilization via nickel affinity matrices. Cross-linked to horseradish peroxidase (HRP). Patent pending.
Species Reactivity:
All
Species Reactivity Note:
All
Application Notes:
Recognizes tyrosine-phosphorylated proteins, useful in immunoblotting.
Control:
A431 carcinoma cells.
Included Positive Antigen Control: Catalog # 12-302, EGF-stimulated A431 cell lysate is provided as a free positive antigen control for western immunoblotting.
Included Positive Antigen Control: Catalog # 12-302, EGF-stimulated A431 cell lysate is provided as a free positive antigen control for western immunoblotting.
Quality Assurance:
Routinely evaluated in western blot to detect tyrosine-phosphorylated proteins in RIPA lysates from EGF-stimulated human A431 carcinoma cells.
Western Blot Analysis:
Tested at 1:2500-1:5000, this lot detected tyrosine-phosphorylated proteins in RIPA lysates from EGF-stimulated human A431 carcinoma cells
Western Blot Analysis:
Tested at 1:2500-1:5000, this lot detected tyrosine-phosphorylated proteins in RIPA lysates from EGF-stimulated human A431 carcinoma cells
Purification Method:
Protein G purfied
Presentation:
IgG2bκ-HRP conjugate in PBS containing 0.05% ProClin®150, as a preservative.
Storage Conditions:
Stable for 6 months at 2-8°C from date of receipt.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: For optimal performance, remove HRP precipitate by centrifuging vial prior to use.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: For optimal performance, remove HRP precipitate by centrifuging vial prior to use.
UniProt Number:
Entrez Gene Number:
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Key Applications:
Western Blotting
Brand Family:
Upstate
Protein/Isoform Description:
Protein Tyrosine phosphorylation was first described in 1979. The development of the Anti-Phosphotyrosine, clone 4G10 in 1989 was a monumental discovery for researchers. 4G10 is the first and best single monoclonal antibody for the detection of tyrosine phosphorylation. It is the most highly cited (75% of related scientific journal articles over the last three years) and validated phosphotyrosine antibody available. It consistently produces high quality, reproducible data, and has been validated by thousands of scientific and medical researchers in virtually every application and tyrosine target over the past 18 years. In addition to its unparalleled characterization and applicability, 4G10 is well known as one of the most sensitive anti-phosphotyrosines. Now Millipore is excited to announce that we have taken the sensitivity for phosphotyrosine detection to the next level. To improve on something that hundreds have tried and none have succeeded, we pooled 4G10 with the next most highly regarded Anti-phosphotyrosine, clone PY20 to make 4G10 Platinum. PY20 itself is a very poor substitute for 4G10, but its additive effect allows for a greater level of detection on more substrates that even 4G10 alone was capable of.
Product Name:
Anti-Phosphotyrosine, recombinant clone 4G10®, HRP conjugate
Antibody Type:
Monoclonal Antibody
Qty/Pk:
10 blots
Format:
HRP
Host:
Mouse