Ordering Information
- : 16-199
- : 1 mg
- : 1 mg antibody covalently coupled to 1 mL protein A agarose beads for a total volume of 2 mL.
Product Images
WB: Phosphotyrosine containing proteins were immunoprecipitated from 500 micrograms of an EGF-stimulated cell lysate using 10 microliters of Anti-pho...
Anti-Phosphotyrosine Antibody, recombinant clone 4G10®, agarose conjugate
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | IP | Mouse | Gel Immobilized | Monoclonal Antibody |
Description:
Anti-Phosphotyrosine Antibody, recombinant clone 4G10®, agarose conjugate
Promotional Text:
Trade Name:
Upstate (Millipore)
Specificity:
Recognizes tyrosine-phosphorylated proteins from all species.
Molecular Weight:
Dependent upon the molecular weight of the tyrosine phosphorylated protein being detected.
Epitope:
N/A
Modifications:
Phosphorylation
Clone:
4G10®
Background Information:
Some of the tyrosine residues can be tagged with a phosphate group (phosphorylated) by protein kinases. (In its phosphorylated state, it is referred to as phosphotyrosine.). Tyrosine phosphorylation is considered as one of the key steps in signal transduction and regulation of enzymatic activity. The advent of anti-phospho-tyrosine antibodies is one of significant events in signal transduction research. Before the availability of anti-phosphotyrosine antibodies, tyrosyl phospyhorylation of proteins and enzymes was investigated through hazardous and time-consuming radioactive experiments. Anti-phosphotyrosine antibodies are commonly used in western blots after the targeted proteins have been immunoprecipitated to measure the tyrosyl phosphorylation of the proteins. Anti-phosphotyrosine antibodies are also directly used on cell lysate to examine the overall change of tyrosine phosphorylation level in reponse to various treatments.
Species Reactivity:
Human
Application Notes:
This product is useful for immunoprecipitation and immunoaffinity chromatography methods.
Control:
Pervanadate-treated human A431 cell extracts, EGF-treated human A431 cells.
Quality Assurance:
Routinely evaluated by immunoprecipitating the EGF receptor and other tyrosine phosphorylated proteins from EGF-stimulated A431 cells.
Immunoprecipitation Analysis:
10 μL of this lot immunoprecipitated the EGF receptor and other tyrosine phosphorylated proteins from EGFstimulated A431 cells.
Immunoprecipitation Analysis:
10 μL of this lot immunoprecipitated the EGF receptor and other tyrosine phosphorylated proteins from EGFstimulated A431 cells.
Purification Method:
Protein A purfied
Presentation:
Gel Immobilized mouse monoclonal antibody covalently coupled to protein A agarose beads and provided as a 50% slurry suspended in PBS, containing 0.01% Kathon, as a preservative for a total volume of 2 mL. Liquid suspension.
Storage Conditions:
Stable for 6 months at 2-8ºC from date of receipt.
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Key Applications:
Immunoprecipitation
Brand Family:
Upstate
Product Name:
Anti-Phosphotyrosine Antibody, recombinant clone 4G10®, agarose conjugate
Concentration:
1 mg antibody covalently coupled to 1 mL protein A agarose beads for a total volume of 2 mL.
Antibody Type:
Monoclonal Antibody
Qty/Pk:
1 mg
Format:
Gel Immobilized
Host:
Mouse