Ordering Information
- : 16-204
- : 100 µg
- : 1 mg/mL
Product Images
WB: EGF-stimulated A431 cell lysate was probed with anti-phosphotyrosine, recombinant 4G10®, biotin conjugate.
Anti-Phosphotyrosine Antibody, recombinant clone 4G10®, biotin conjugate
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| A | WB | Mouse | Biotin | Monoclonal Antibody |
Description:
Anti-Phosphotyrosine Antibody, recombinant clone 4G10®, biotin conjugate
Promotional Text:
Trade Name:
Upstate (Millipore)
Product Overview:
Included Positive Antigen Control: Catalog # 12-302, EGF-stimulated A431 cell lysate
Product Description: Produced from CHO cells expressing the 4G10 antibody heavy and light chain cDNAs. Heavy chain C-terminus has a hexa-histidine tag for purification and immobilization via nickel affinity matrices. Cross-linked to biotin. Patent pending.
Product Description: Produced from CHO cells expressing the 4G10 antibody heavy and light chain cDNAs. Heavy chain C-terminus has a hexa-histidine tag for purification and immobilization via nickel affinity matrices. Cross-linked to biotin. Patent pending.
Specificity:
Tyrosine-phosphorylated proteins from all species.
Molecular Weight:
Dependent upon the molecular weight of the tyrosine phosphorylated protein being detected.
Epitope:
N/A
Immunogen:
Phosphotyramine-KLH
Modifications:
Phosphorylation
Clone:
4G10®
Isotype:
IgG2bκ
Background Information:
Produced from CHO cells expressing the 4G10 antibody heavy and light chain cDNAs. Heavy chain C-terminus has a hexa-histidine tag for purification and immobilization via nickel affinity matrices. Cross-linked to biotin. Patent pending.
Species Reactivity:
All
Control:
A431 carcinoma cells.
Included Positive Antigen Control: Catalog # 12-302, EGF-stimulated A431 cell lysate is provided as a free positive antigen control for western immunoblotting.
Included Positive Antigen Control: Catalog # 12-302, EGF-stimulated A431 cell lysate is provided as a free positive antigen control for western immunoblotting.
Quality Assurance:
Routinely evaluated by western blot to detect tyrosine phosphorylated proteins in a RIPA lysate of EGF-stimulated A431 carcinoma cells.
Western Blot Analysis:
0.5-2 μg/mL of this lot detected tyrosine phosphorylated proteins in a RIPA lysate of EGF-stimulated A431 carcinoma cells.
Western Blot Analysis:
0.5-2 μg/mL of this lot detected tyrosine phosphorylated proteins in a RIPA lysate of EGF-stimulated A431 carcinoma cells.
Purification Method:
Protein G purfied
Presentation:
Biotin-conjugated recombinant mouse IgG2bκ in PBS containing 0.05% sodium azide. Liquid at 2-8°C.
Storage Conditions:
Stable for 9 months at 2-8ºC from date of receipt.
For maximum recovery of product, centrifuge the vial prior to removing the cap. DO NOT FREEZE!
For maximum recovery of product, centrifuge the vial prior to removing the cap. DO NOT FREEZE!
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Key Applications:
Western Blotting
Brand Family:
Upstate
Product Name:
Anti-Phosphotyrosine Antibody, recombinant clone 4G10®, biotin conjugate
Concentration:
1 mg/mL
Antibody Type:
Monoclonal Antibody
Qty/Pk:
100 µg
Format:
Biotin
Host:
Mouse