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IB: Acid extracts from untreated (Lane 1) and colcemid treated (Lane 2) HeLa cells were resolved by electrophoresis, transferred to nitrocellulose and...
Anti-Phospho-Histone H3 (Ser10), clone 3H10, Cy5 conjugate
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | IF, WB | Mouse | Purified | Monoclonal Antibody |
UniProt Number:
UniProt Summary
FUNCTION: SwissProt: Q16695 # Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
SIZE: 136 ami ... see more »
SIZE: 136 ami ... see more »
Entrez Gene Number:
Entrez Gene Summary
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member
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Description:
Anti-Phospho-Histone H3 (Ser10), clone 3H10, Cy5 conjugate
Background Information:
Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the 'beads on a string' structure.
The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.
The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.
Specificity:
Histone H3 phosphorylated at Ser10
Key Applications:
- Immunofluorescence
- Western Blotting
Species Reactivity:
Human
Species Reactivity Note:
Expected to cross react with all vertebrate species.
Isotype:
IgG1κ
Immunogen:
A proprietary immunogen based on a peptide sequence containing phospho-serine corresponding to residue 10 of human histone H3, clone 3H10. This is a Cy5 conjugate of Anti-phospho-Histone H3 (Ser10), clone 3H10, Catalog # 05-806.
Modifications:
Phosphorylation
Molecular Weight:
17kDa
Control:
Acid extracts from colcemid treated HeLa cells (lane 2)
Storage Conditions:
1 year at 4°C from date of shipment
Gene Symbol:
- HIST3H3
- H3FT
- H3/t
- H3t
- MGC126888
- H3/g
- H3.4
- H3T
- MGC126886
Quality Assurance:
Routinely evaluated by immunoblot in acid extrated protein from mitotic HeLa cells treated with colcemid (17-306).
Antibody Category:
Epigenetics & Nuclear Function
Antibody Sub-Category:
Histones
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Trade Name:
Upstate (Millipore)
Antibody Type:
Monoclonal Antibody
Format:
Purified
Host:
Mouse
Purification Method:
protein G purified