Product Images
ICC: HeLa cells were stained with 1μg/ml of Anti-Phospho-Ser/Thr-Pro, MPM-2, Cy5 Conjugate (red) and DAPI (blue).
WB: Untreated HeLa (lane 1) or colcemid-treated HeLa (lane 2) cell lysates were resolved by electophoresis, transferred to nitrocellulose and probed w...
Anti-phospho-Ser/Thr-Pro MPM-2 Antibody, Cy5 conjugate
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| Vrt | IF, WB, ICC | Mouse | Purified | Monoclonal Antibody |
Description:
Anti-phospho-Ser/Thr-Pro Antibody, MPM-2, Cy5 conjugate | 16-220
Promotional Text:
Trade Name:
Upstate (Millipore)
Product Overview:
Specificity:
Recognizes a phosphorylated epitope (phospho-[Ser/Thr]Pro) found in phosphoproteins such as MAP2, HSP70, cdc25, and DNA topoisomerase IIα, most of which are phosphorylated at the onset of mitosis. The number of phosphoproteins recognized by MPM-2 varies from species to species and with the cell type.
Immunogen:
Mitotic human HeLa cell lysate
Modifications:
Phosphorylation
Isotype:
IgG1
Background Information:
Progression of cells from interphase to mitosis involves alterations in cell structures and activities. The transition from G2 to M phase is induced by M phase-promoting factor, or MPF. In M phase, many proteins are phosphorylated directly by MPF or indirectly by kinases activated by MPF. These M-phase phosphoproteins (MPPs, or MPHOSPHs) permit disassembly of interphase structures and generation of M-phase enzymatic activities and structures. Also, known as Forkhead-related protein FKHL16 or Hepatocyte nuclear factor 3 forkhead homolog 11.
Species Reactivity:
Vertebrates
Control:
Colcemid-treated HeLa cell lysates
Quality Assurance:
Routinely evaluated by immunoblot on colcemid-treated HeLa cell lysates.
Purification Method:
Protein G purfied
Presentation:
100μg Cy5-conjugated mouse IgG1 in 200μl of PBS containing 1% BSA, 0.05% Tween®-20, 0.05% sodium azide.
Storage Conditions:
1 year at 4°C from date of shipment
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Key Applications:
- Immunofluorescence
- Western Blotting
- Immunocytochemistry
Brand Family:
Upstate
Product Name:
Anti-phospho-Ser/Thr-Pro Antibody, MPM-2, Cy5 conjugate | 16-220
Antibody Type:
Monoclonal Antibody
Qty/Pk:
100 µg
Format:
Purified
Host:
Mouse