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Immunoblot Analysis
20μg of non-stimulated (lane 1) and peroxynitrite-treated (lane 2) A431 cell lysates were nitrocellulose and probed with 2μ...
Anti-Nitrotyrosine, clone 1A6, Alexa Fluor® 488 conjugate
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| Vrt | FC, WB | Mouse | AlexaFluor®488 | Monoclonal Antibody |
Description:
Anti-Nitrotyrosine, clone 1A6, Alexa Fluor® 488 conjugate
Trade Name:
Upstate (Millipore)
Specificity:
Recognizes and is specific for recombinant proteins containing the Myc epitope tag (EQKLISEEDL) in a variety of sequence contexts. Also recognizes human Myc.
Immunogen:
Nitrated KLH
Clone:
1A6
Isotype:
IgG2bκ
Species Reactivity:
Vertebrates
Control:
Nitrated BSA
Quality Assurance:
Routinely evaluated by immunoblot.
Purification Method:
Protein G purfied
Presentation:
Protein G Purified mouse monoclonal in a solution of 1% BSA, PBS, 0.05% Tween, and 0.05% sodium azide as a preservative.
Storage Conditions:
Stable for 1 year at 2–8°C from date of shipment in undiluted aliquots. Upon receipt, and prior to removing the cap, centrifuge the vial to remove any material in the cap. DO NOT FREEZE. Store at 2–8°C in aliquots, in the dark. Avoid repeated warming/cooling cycles.
Usage Statement:
- Alexa Fluor® is a registered trademark of Molecular Probes, Inc.
- Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Key Applications:
- Flow Cytometry
- Western Blotting
Qty/Pk:
100 μg
Brand Family:
Upstate
Format:
AlexaFluor®488
Host:
Mouse
Product Name:
Anti-Nitrotyrosine, clone 1A6, Alexa Fluor® 488 conjugate
Concentration:
0.5 mg/mL
Antibody Type:
Monoclonal Antibody