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Immunocytochemistry: A431 cells were treated (Panel A) with EGF at 1μg/ml for 10 minutes or untreated (Panel B) and and stained with 2μg/ml of anti-ph...
Anti-phospho-Src (Tyr416), clone 9A6, Alexa Fluor® 488 conjugate
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M, R | FC, IF, IC | Mouse | AlexaFluor®488 | Monoclonal Antibody |
Description:
Anti-phospho-Src (Tyr416), clone 9A6, Alexa Fluor® 488 conjugate
Trade Name:
Upstate (Millipore)
Specificity:
phospho-Src
Molecular Weight:
60kDa
Immunogen:
KLH-conjugated, synthetic peptide containing phosphotyrosine in the sequence context corresponding to tyrosine 416 of avian Src
Modifications:
Phosphorylation
Clone:
9A6
Isotype:
IgG1κ
Species Reactivity:
- Human
- Mouse
- Rat
Quality Assurance:
Routinely evaluated by positive immunostaining for phospho-Src in A431 cells.
Storage Conditions:
1 year at 4°C from date of shipment
UniProt Number:
Entrez Gene Number:
Gene Symbol:
- SRC
- ASV
- c-src
- p60-Src
- SRC1
- c-SRC
- pp60c-src
Usage Statement:
- Alexa Fluor® is a registered trademark of Molecular Probes, Inc.
- Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Entrez Gene Summary:
This gene is highly similar to the v-src gene of Rous sarcoma virus. This proto-oncogene may play a role in the regulation of embryonic development and cell growth. The protein encoded by this gene is a tyrosine-protein kinase whose activity can be inhibited by phosphorylation by c-SRC kinase. Mutations in this gene could be involved in the malignant progression of colon cancer. Two transcript variants encoding the same protein have been found for this gene.
Key Applications:
- Flow Cytometry
- Immunofluorescence
- Immunocytochemistry
UniProt Summary:
SIZE: 536 amino acids; 59835 Da
SUBUNIT: Interacts with DDEF1/ASAP1 via its SH3 domain. Interacts with CCPG1 (By similarity). Interacts with CDCP1, PELP1, TGFB1I1 and TOM1L2.
PTM: Phosphorylated on Tyr-530 by c-Src kinase (CSK). The phosphorylated form is termed pp60c-src. The phosphorylated tail interacts with the SH2 domain thereby repressing kinase activity.
SIMILARITY: SwissProt: P12931 ## Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily. & Contains 1 protein kinase domain. & Contains 1 SH2 domain. & Contains 1 SH3 domain.
SUBUNIT: Interacts with DDEF1/ASAP1 via its SH3 domain. Interacts with CCPG1 (By similarity). Interacts with CDCP1, PELP1, TGFB1I1 and TOM1L2.
PTM: Phosphorylated on Tyr-530 by c-Src kinase (CSK). The phosphorylated form is termed pp60c-src. The phosphorylated tail interacts with the SH2 domain thereby repressing kinase activity.
SIMILARITY: SwissProt: P12931 ## Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily. & Contains 1 protein kinase domain. & Contains 1 SH2 domain. & Contains 1 SH3 domain.
Qty/Pk:
25 µg
Brand Family:
Upstate
Format:
AlexaFluor®488
Host:
Mouse
Product Name:
Anti-phospho-Src (Tyr416), clone 9A6, Alexa Fluor® 488 conjugate
Antibody Type:
Monoclonal Antibody