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Left panel: HL60 cells stained with anti-phospho-Histone H2B (Ser14), clone MC603, FITC conjugate after overnight serum starvation, negative co...

Anti-phospho-Histone H2B (Ser14) Antibody, clone MC603, FITC conjugate, rabbit monoclonal

Species Reactivity Key Applications Host Format Antibody Type
H FC Rabbit Purified Monoclonal Antibody
Description:
Anti-phospho-Histone H2B (Ser14) Antibody, clone MC603, FITC conjugate, rabbit monoclonal
Promotional Text:
Trade Name:
Upstate (Millipore)
Specificity:
phospho-histone H2B (Ser14)
Molecular Weight:
15kDa
Immunogen:
KLH-conjugated synthetic peptide containing the sequence KGpSK in which pS corresponds to phospho-serine 14 of human Histone H2B. Clone MC603.
Modifications:
Phosphorylation
Clone:
MC603
Isotype:
Species Reactivity:
Human
Species Reactivity Note:
Broad species cross-reactivity expected based on sequence homology.
Quality Assurance:
Routinely evaluated by flow cytometry.
Purification Method:
Protein A purfied
Storage Conditions:
2 years at 4°C from date of shipment
UniProt Number:
Entrez Gene Number:
Gene Symbol:
HIST3H2BB
Alternate Names:
  • H2BS14P
  • Histone H2B (phospho S14)
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Key Applications:
Flow Cytometry
Entrez Gene Summary:
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H2B family. Transcripts from this gene contain a palindromic termination element.
UniProt Summary:
FUNCTION: SwissProt: Q8N257 # Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
SIZE: 126 amino acids; 13908 Da
SUBUNIT: The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.
SUBCELLULAR LOCATION: Nucleus.
PTM: Monoubiquitination of Lys-121 by the RNF20/40 complex gives a specific tag for epigenetic transcriptional activation and is also prerequisite for histone H3 'Lys-4' and 'Lys-79' methylation. It also functions cooperatively with the FACT dimer to stimulate elongation by RNA polymerase II. & Phosphorylated on Ser-15 by STK4/MST1 during apoptosis; which facilitates apoptotic chromatin condensation. Also phosphorylated on Ser-15 in response to DNA double strand breaks (DSBs), and in correlation with somatic hypermutation and immunoglobulin class- switch recombination.
SIMILARITY: SwissProt: Q8N257 ## Belongs to the histone H2B family.
Brand Family:
Upstate
Product Name:
Anti-phospho-Histone H2B (Ser14) Antibody, clone MC603, FITC conjugate, rabbit monoclonal
Antibody Type:
Monoclonal Antibody
Qty/Pk:
50 µg
Format:
Purified
Host:
Rabbit
Applications:
Anti-phospho-Histone H2B (Ser14) Antibody, clone MC603, FITC conjugate is a rabbit monoclonal antibody for detection of phospho-Histone H2B (Ser14) also known as H2BS14P, Histone H2B (phospho S14) & has been validated in FC.

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