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IP/Western Blot Analysis:
Representative lot data.
100 μg of NIH3T3 whole cell lysate stimulated with 100ng/mL PDGF (10 minutes) was immunop...
IP/Western Blot Analysis:
Representative lot data.
100 μg of NIH3T3 whole cell lysate stimulated with 100ng/mL PDGF (10 minutes) was immunop...
Anti-Phosphotyrosine, clone 4G10®, Magnetic Bead Conjugate
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| A | IP | Mouse | Purified | Monoclonal Antibody |
Description:
Anti-Phosphotyrosine Antibody, clone 4G10®, Magnetic Bead Conjugate | 16-282
Promotional Text:
Specificity:
This antibody recognizes tyrosine-phosphorylated proteins from all species
Molecular Weight:
Dependent upon the molecular weight of the tyrosine phosphorylated protein being detected.
Immunogen:
Phosphotyramine-KLH
Isotype:
IgG2bκ
Background Information:
Tyrosine phosphorylation is considered as one of the key steps in signal transduction and regulation of enzymatic activity. Some of the tyrosine residues can be tagged with a phosphate group (phosphorylated) by protein kinases. (In its phosphorylated state, it is referred to as phosphotyrosine.).The advent of anti-phosphotyrosine antibodies is one of significant events in signal transduction research. Before the availability of anti-phosphotyrosine antibodies, tyrosyl phospyhorylation of proteins and enzymes was investigated through hazardous and time-consuming radioactive experiments. Anti-phosphotyrosine antibodies are commonly used in western blots after the targeted proteins have been immunoprecipitated to measure the tyrosyl phosphorylation of the proteins. Anti-phosphotyrosine antibodies are also directly used on cell lysate to examine the overall change of tyrosine phosphorylation level in reponse to various treatments.
Species Reactivity:
All
Species Reactivity Note:
All
Control:
NIH3T3 whole cell lysate stimulated with 50 ng/mL PDGF (5 minutes)
Quality Assurance:
100 μg of NIH3T3 whole cell lysate stimulated with 100 ng/mL PDGF (10 minutes) was immunoprecipitated (IP) using either anti-mouse IgG (Cat. No. 12-371) (lane 1), or Anti-Phosphotyrosine, Magnetic Bead Conjugate
(Lane 2).
(Lane 2).
Purification Method:
Protein G-Sepharose Chromatography
Presentation:
20% slurry; 1xPBS, 0.05% sodium azide. Sufficient for 10 IPs.
Storage Conditions:
Stable for 1 year at 2-8ºC from date of receipt.
Note: DO NOT FREEZE
Note: DO NOT FREEZE
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Key Applications:
Immunoprecipitation
Qty/Pk:
200 μL
Format:
Purified
Host:
Mouse
Product Name:
Anti-Phosphotyrosine, clone 4G10®, Magnetic Bead Conjugate
Antibody Type:
Monoclonal Antibody