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Ordering Information
- : 16-305
- : 50 µg
Product Images
2µg/ml of this lot showed positive immunostaining for phosphorylated Akt1/PKBα in 3T3 cells treated with PDGF (100ng/mL, 20min.).
Anti-phospho-Akt1/PKBα (Ser473), clone 11E6, Alexa Fluor® 488 conjugate
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| Ca, H, M, R | FC, IC, IF, WB | Mouse | Purified | Monoclonal Antibody |
Description:
Anti-phospho-Akt1/PKBα (Ser473), clone 11E6, Alexa Fluor® 488 conjugate
Trade Name:
Upstate (Millipore)
Specificity:
Recognizes phosphorylatedAkt1/PKBα at Ser473. This lot of antibody does not cross-react with Akt1/PKBα dephosphorylated at Ser473.
Molecular Weight:
60 kDa
Immunogen:
KLH conjugated synthetic peptide containing a pSer that corresponds to amino acid residues around phosphoserine 473.
Modifications:
Phosphorylation
Clone:
11E6
Isotype:
IgG1
Species Reactivity:
- Canine
- Human
- Mouse
- Rat
Quality Assurance:
Routinely evaluated by immunocytochemistry and flow cytometry.
Purification Method:
Purified from serum-free cell culture supernatant by subsequent thiophilic adsorption and size exclusion chromatography
Storage Conditions:
1 year at 4°C from date of shipment
UniProt Number:
Entrez Gene Number:
Gene Symbol:
- AKT1
- RAC
- PRKBA
- MGC99656
- RAC-ALPHA
- RAC-PK-alpha
- C-AKT
- PKB
- AKT
Alternate Names:
PKB, PKD-alpha, AKT1, Protein Kinase B
Usage Statement:
- Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
- Alexa Fluor® is a registered trademark of Molecular Probes, Inc.
Key Applications:
- Flow Cytometry
- Immunocytochemistry
- Immunofluorescence
- Western Blotting
Entrez Gene Summary:
The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Multiple alternatively spliced transcript variants have been found for this gene.
UniProt Summary:
FUNCTION: SwissProt: P31749 # General protein kinase capable of phosphorylating several known proteins. Phosphorylates TBC1D4. Signals downstream of phosphatidylinositol 3-kinase (PI(3)K) to mediate the effects of various growth factors such as platelet-derived growth factor (PDGF), epidermal growth factor (EGF), insulin and insulin-like growth factor I (IGF-I). Plays a role in glucose transport by mediating insulin-induced translocation of the GLUT4 glucose transporter to the cell surface. Mediates the antiapoptotic effects of IGF-I. Mediates insulin-stimulated protein synthesis, partly by playing a role in both insulin-induced phosphorylation of 4E-BP1 and in insulin-induced activation of p70 S6 kinase. Promotes glycogen synthesis by mediating the insulin-induced activation of glycogen synthase.
SIZE: 480 amino acids; 55686 Da
SUBUNIT: Interacts with CENTG1 isoform 2 (PIKE-A) in the presence of guanine nucleotides. The C-terminus interacts with CCDC88A/GRDN. Interacts with AKTIP.
SUBCELLULAR LOCATION: Cytoplasm. Nucleus. Note=Nucleus after activation by integrin-linked protein kinase 1 (ILK1).
TISSUE SPECIFICITY: In all human cell types so far analyzed.
DOMAIN: SwissProt: P31749 Binding of the PH domain to the phosphatidylinositol 3- kinase alpha (PI(3)K) results in its targeting to the plasma membrane.
PTM: Phosphorylation on Thr-308, Ser-473 and Tyr-474 is required for full activity. Ser-473 phosphorylation by the Rictor-mTor complex favors Thr-308 phosphorylation by PDPK1. Ser-473 phosphorylation is enhanced by interaction with CENTG1 isoform 2 (PIKE-A).
SIMILARITY: Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. RAC subfamily. & Contains 1 AGC-kinase C-terminal domain. & Contains 1 PH domain. & Contains 1 protein kinase domain.
SIZE: 480 amino acids; 55686 Da
SUBUNIT: Interacts with CENTG1 isoform 2 (PIKE-A) in the presence of guanine nucleotides. The C-terminus interacts with CCDC88A/GRDN. Interacts with AKTIP.
SUBCELLULAR LOCATION: Cytoplasm. Nucleus. Note=Nucleus after activation by integrin-linked protein kinase 1 (ILK1).
TISSUE SPECIFICITY: In all human cell types so far analyzed.
DOMAIN: SwissProt: P31749 Binding of the PH domain to the phosphatidylinositol 3- kinase alpha (PI(3)K) results in its targeting to the plasma membrane.
PTM: Phosphorylation on Thr-308, Ser-473 and Tyr-474 is required for full activity. Ser-473 phosphorylation by the Rictor-mTor complex favors Thr-308 phosphorylation by PDPK1. Ser-473 phosphorylation is enhanced by interaction with CENTG1 isoform 2 (PIKE-A).
SIMILARITY: Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. RAC subfamily. & Contains 1 AGC-kinase C-terminal domain. & Contains 1 PH domain. & Contains 1 protein kinase domain.
Brand Family:
Upstate
Product Name:
Anti-phospho-Akt1/PKBα (Ser473), clone 11E6, Alexa Fluor® 488 conjugate
Antibody Type:
Monoclonal Antibody
Qty/Pk:
50 µg
Format:
Purified
Host:
Mouse