Product Images
Western Blot Analysis:
Insulin-stimulated HEK293 cell lysate was resolved by electrophoresis, transferred to PVDF and probed with 4G10® Platinum,...
ELISA analysis: Representative data showing ELISA performed using 1:1000 of 4G10® Platinum HRP (Cat. # 16-316) on a plate coated with a serial dilutio...
Anti-Phosphotyrosine Antibody, 4G10® Platinum, HRP Conjugate
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| A | WB, ELISA | Mouse | HRP | Monoclonal Antibody |
Description:
Anti-Phosphotyrosine Antibody, 4G10® Platinum, HRP Conjugate | 16-316
Promotional Text:
Trade Name:
Upstate (Millipore)
Specificity:
Tyrosine-phosphorylated proteins from all species.
Molecular Weight:
Varies
Immunogen:
4G10® used Phosphotyramine coupled to KLH and PY20 used phosphotyrosine conjugated to carrier protein
Modifications:
Phosphorylation
Clone:
4G10® and PY20
Isotype:
IgG2b
Background Information:
The development of the anti-phosphotyrosine, clone 4G10® in 1989 was a monumental discovery for researchers. 4G10® was the first and is the best single monoclonal antibody for the detection tyrosine phosphorylation. 4G10® is well known for its sensitivity and its ability to detect multiple tyrosine phosphorylations on numerous substrates. It has been validated by thousands of scientific and medical researchers in virtually every application and tyrosine target over the past 2 decades. To improve on something that hundred have tried and no one has succeeded, we pooled 4G10® with the next most highly regarded anti-phosphotyrosine, clone PY20 to make 4G10® Platinum. PY20 itself is a very poor substitute for 4G10®, but its additive effect allow for a greater level of detection on more substrates that even 4G10® alone.
Species Reactivity:
All
Quality Assurance:
Routinely evaluated by western blot.
Purification Method:
Protein A purfied
Presentation:
100 μL of a proprietary mixture of protein G purified mouse monoclonal 4G10® (IgG2bƙ) HRP conjugate and protein A purified mouse monoclonal PY20, HRP conjugate (IgG2b) in PBS containing 0.05% ProClin®150 and HRP stabilizing compounds. Sufficient for a minumum of 10 immunoblots, if used at the optimum concentration of 1:2000 – 1:4000 in 10 mL blocking buffer. Liquid.
Storage Conditions:
6 months at 2-8°C from date of shipment.
NOTE: DO NOT FREEZE. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
NOTE: DO NOT FREEZE. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
UniProt Number:
Entrez Gene Number:
Alternate Names:
phosphotyrosine
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Key Applications:
- Western Blotting
- ELISA
Brand Family:
Upstate
Product Name:
4G10® Platinum, Anti-Phosphotyrosine, HRP Conjugate
Antibody Type:
Monoclonal Antibody
Qty/Pk:
100 μL
Format:
HRP
Host:
Mouse