Product Images
Western Blot Analysis:
Insulin-stimulated HEK293 cell lysate was resolved by electrophoresis, transferred to PVDF and probed with PY20, HRP Conju...
ELISA analysis: Representative data showing ELISA performed using 1:1000 of Anti-Phosphotyrosine, clone PY20, HRP Conjugate (Cat.# 16-454) on a plate ...
Anti-Phosphotyrosine Antibody, clone PY20, HRP Conjugate
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| A | WB, ELISA | Mouse | HRP | Monoclonal Antibody |
Description:
Anti-Phosphotyrosine Antibody, clone PY20, HRP Conjugate | 16-454
Promotional Text:
Trade Name:
Upstate (Millipore)
Molecular Weight:
Varies
Epitope:
phosphotyrosine
Immunogen:
Phosphotyrosine conjugated to carrier protein
Modifications:
Phosphorylation
Clone:
PY20
Isotype:
IgG2b
Background Information:
Protein tyrosine phosphorylation was first described in 1979. Large numbers of tyrosine kinases have been described since, and many of the signaling pathways initiated by tyrosine phosphorylation have been delineated. Availability of a top-quality anti-phosphotyrosine antibody has allowed the study of tyrosine kinase activity by simple blotting procedures, and without routine need of large quantities of radioactivity. The 4G10® monoclonal anti-phosphotyrosine antibody (Cat. # 05-321) has been widely recognized as a highly specific and sensitive. This antibody is suitable for all applications involving protein tyrosine phosphorylation. The slightly less sensitive, but still highly regarded clone PY20 can be used as an alternative to the 4G10® product line.
Species Reactivity:
All
Species Reactivity Note:
Recognizes proteins containing phosphotyrosine residues, regardless of origin.
Quality Assurance:
Routinely evaluated by western blot on RIPA lysates from insulin-treated human HEK293 cells.
Purification Method:
Protein A purfied
Presentation:
100 μL of protein A purified mouse IgG2b in PBS containing 0.05% ProClin®150 and HRP stabilizing compounds. Sufficient for a minimum of 10 immunoblots, if used at the optimum concentration of 1:2000- 1:4000 in 10 mL blocking buffer. Liquid.
Storage Conditions:
6 months at 2-8°C from date of shipment.
NOTE: DO NOT FREEZE. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
NOTE: DO NOT FREEZE. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
UniProt Number:
Entrez Gene Number:
Alternate Names:
- PY20
- anti-phosphotyrosine antibody
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Key Applications:
- Western Blotting
- ELISA
Brand Family:
Upstate
Product Name:
Anti-Phosphotyrosine, clone PY20, HRP Conjugate
Antibody Type:
Monoclonal Antibody
Qty/Pk:
10 blots
Format:
HRP
Host:
Mouse