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Immunoprecipitation Analysis:
Representative lot data.
Phosphotyrosine containing proteins were immunoprecipitated from PDGF-stimulated NIH3...
4G10® Platinum, Anti-Phosphotyrosine Antibody, Agarose Conjugate
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| A | IP | Mouse | Purified | Monoclonal Antibody |
Description:
4G10® Platinum, Anti-Phosphotyrosine, Agarose Conjugate
Promotional Text:
Specificity:
Tyrosine-phosphorylated proteins from all species.
Molecular Weight:
Dependent upon the molecular weight of the Tyrosine-phosphorylated protein
Epitope:
phosphotyrosine
Immunogen:
The immunogen for 4G10® was phospho-tyramine coupled to KLH, while the immunogen for PY20 was phosphotyrosine conjugated to a carrier protein.
Clone:
4G10®
Isotype:
IgG2bκ
Background Information:
The development of the anti-phosphotyrosine, clone 4G10® in 1989 was a monumental discovery for researchers. 4G10® was the first and best single monoclonal antibody for the detection tyrosine phosphorylation. 4G10® is well known for its sensitivity and its ability to detect multiple tyrosine phosphorylations on numerous substrates. It has been validated by thousands of scientific and medical researchers in virtually every application and tyrosine target over the past 2 decades. Now Millipore has made the best even better with 4G10® Platinum. We pooled 4G10® with the next most highly regarded anti-phosphotyrosine, clone PY20, to make 4G10® Platinum. PY20 itself is a very poor substitute for 4G10®, but its additive effect allow for a greater level of detection on more substrates that even 4G10® alone is capable.
Species Reactivity:
All
Quality Assurance:
Immunoprecipitation:
Twenty μl of 50% slurry of this reagent (10 μl packed beads) was used to immunoprecipitate tyrosine-phosphorylated proteins from 250 μg PDGF-stimulated NIH3T3 cell lysate or EGF-stimulated A431 cell lysate.
Twenty μl of 50% slurry of this reagent (10 μl packed beads) was used to immunoprecipitate tyrosine-phosphorylated proteins from 250 μg PDGF-stimulated NIH3T3 cell lysate or EGF-stimulated A431 cell lysate.
Purification Method:
protein G purified mouse monoclonal 4G10® (IgG2bκ) and protein A purified PY20 (IgG2b)
Presentation:
Supplied as a 50% slurry of a proprietary mixture of agarose conjugated, protein G purified mouse monoclonal 4G10® (IgG2bκ) and protein A purified PY20 (IgG2b) in PBS, pH 7.4 with 0.05% sodium azide.
Storage Conditions:
Stable for 6 months at 2-8°C from date of shipment. For maximum recovery of product, centrifuge the vial prior to removing the cap.
NOTE: DO NOT FREEZE.
NOTE: DO NOT FREEZE.
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Key Applications:
Immunoprecipitation
Product Name:
4G10® Platinum, Anti-Phosphotyrosine, Agarose Conjugate
Antibody Type:
Monoclonal Antibody
Qty/Pk:
200 μL
Format:
Purified
Host:
Mouse
Applications:
4G10® Platinum, Anti-Phosphotyrosine, Agarose Conjugate detects levels of Phosphotyrosine proteins and has been published and validated for use in IP.