日本注文に関する情報
- : 17-10085
- : 22 assays
Magna ChIP™ A/G Chromatin Immunoprecipitation Kit
Description:
Magna ChIP™ A/G Chromatin Immunoprecipitation Kit
Trade Name:
Upstate (Millipore)
Qty/Pk:
22 assays
Product Overview:
Features & Benefits:
Chromatin Immunoprecipitation (ChIP) is an important technique allowing the analysis of in vivo interactions of proteins with genomic DNA. Any chromatin-associated or DNA binding protein can be analyzed with this technique, provided a good antibody to the protein exists. One can measure different proteins localized to a specific region of the genome, or the genome wide distribution of a specific protein. Another powerful application of this technique is to analyze changes in histone modifications that correlate with processes like transcription, mitosis or DNA repair.
- Magnetic bead-based rapid protocol allows performance of ChIP in as little as 1 day
- Blend of protein A+G bead blend allows ChIP using a broader range of antibodies than A or G alone
- Includes spin columns to make DNA purification easier and more reliable - no more messy phenol-chloroform extractions
- Complete kit with all required reagents for reliable and reproducible results
- Compatible with ChIPAb+ validated antibody and primer sets
Chromatin Immunoprecipitation (ChIP) is an important technique allowing the analysis of in vivo interactions of proteins with genomic DNA. Any chromatin-associated or DNA binding protein can be analyzed with this technique, provided a good antibody to the protein exists. One can measure different proteins localized to a specific region of the genome, or the genome wide distribution of a specific protein. Another powerful application of this technique is to analyze changes in histone modifications that correlate with processes like transcription, mitosis or DNA repair.
Key Applications:
Chromatin Immunoprecipitation (ChIP)
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Components:
- Magnetic Protein A/G Beads
- ChIP Dilution Buffer
- Low Salt Wash Buffer
- High Salt Wash Buffer
- LiCl Wash Buffer
- TE Buffer
- Cell Lysis Buffer
- Nuclear Lysis Buffer
- ChIP Elution Buffer (w/o Proteinase K)
- Proteinase K
- RNase A
- 10X Glycine
- 10X PBS
- Protease Inhibitor Cocktail II
- Spin Filters
- Collection Tubes
- Bind Reagent A
- Wash Reagent B
- Elution Reagent C
Brand Family:
Upstate
Presentation:
Two boxes containing all necessary reagents to perform 22 individual chromatin immunoprecipitation (ChIP) reactions. Supplied buffers are sufficient to generate chromatin from up to five 15 cm plates of cultured cells, each plate providing up to 10 chromatin preparations (varies with cell and assay type).
Storage Conditions:
Upon receipt, store components at the temperatures indicated on the labels. Kit components are stable for 1 year from date of shipment when stored as directed.
Promotional Text:
Discover our complete selection of Magna ChIP Kits
Magna ChIP™ G Tissue Kit
Magna ChIP-Seq™ Chromatin Immunoprecipitation and Next Generation Sequencing Library Preparation Kit
Magna ChIP2 ™ Universal Chromatin Immunoprecipitation DNA Microarray Kit
Magna ChIP™ G Chromatin Immunoprecipitation Kit
Magna ChIP™ A Chromatin Immunoprecipitation Kit
Magna ChIP™ G Tissue Kit
Magna ChIP-Seq™ Chromatin Immunoprecipitation and Next Generation Sequencing Library Preparation Kit
Magna ChIP2 ™ Universal Chromatin Immunoprecipitation DNA Microarray Kit
Magna ChIP™ G Chromatin Immunoprecipitation Kit
Magna ChIP™ A Chromatin Immunoprecipitation Kit
Packaging:
Kit capacity: 22 chromatin immunoprecipitation assays
Product Name:
Magna ChIP™ A/G Chromatin Immunoprecipitation Kit
Materials Required but Not Delivered:
Magna Grip™ Rack 8 well (Cat.# 20-400) or similar magnetic rack.