Magna ChIP-Seq™ ChIP and NGS Library Preparation Kit

Magna ChIP-Seq™ Chromatin Immunoprecipitation and Next Generation Sequencing Library Preparation Kit

Upstate (Millipore)

10 library constructions

ChIP-Seq facilitates genome-wide profiling of the interactions of transcription factors, modified histones, and other chromatin associated proteins. By combining chromatin immunoprecipitation with next generation sequencing, laboratories can generate high resolution data on protein-DNA interactions across the genome. For laboratories interested in gaining a deeper understanding of epigenetic mechanisms of gene regulation, ChIP-Seq has become an indispensable tool. The Magna ChIP-Seq Kit simplifies this technique by providing a validated set of reagents, easy-to-follow protocols, controls, and in process quality control guidelines to allow virtually any laboratory to conduct genome-wide analysis of DNA-protein interactions and the profiling of epigenetic marks.

• Effective ChIP protocol simplifies production and immunoprecipitation of high quality chromatin
  
• Reliable ChIP-Seq library construction from as little as 1 ng of purified ChIP DNA
  
• Easy-to-follow protocols and in-process quality control guidelines help ensure successful ChIP and library construction in as little as 2 days
  
• Magna ChIP A+G bead blend allows the use of antibodies from virtually any species or class
  
• Enzymes and buffers provided in convenient master mix formulations streamlines library construction
  
• Validate the ChIP procedure using inlcuded positive and negative control antibodies and a control primer set
  
• Proven performance through construction and sequencing of genomic DNA libraries on an Illumina Genome Analyzer II

• ChIP-seq
• Chromatin Immunoprecipitation (ChIP)
• Sample Prep

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Magna ChIP-Seq Kit Functional Validation:
ChIP modules are functionally tested in quantitative chromatin immunoprecipitation reactions and each set of library construction reagents is functionally validated together through construction and sequencing of a genomic DNA library on an Illumina Genome Analyzer II.

• Magna ChIP A/G (2° to 8°C) -Chromatin IP Reagents
• Magna ChIP A/G (-20°C) -Chromatin IP Reagents
• Magna ChIP-Seq Library Construction (-20°C) Reagents

Three modules containing all key reagents for chromatin immunoprecipitation and next generation sequencing library preparation from genomic DNA.

Upon receipt, store components at the temperatures indicated on the labels. Kit components are stable for 6 months from date of shipment when used and stored as directed.

Magna ChIP-Seq™ Chromatin Immunoprecipitation and Next Generation Sequencing Library Preparation Kit

Cells, stimulated or treated as desired
Antibody for chromatin immunoprecipitation)
37% Formaldehyde
Taq DNA polymerase and dNTPs
Master Mix for qPCR
DNase and RNase-free sterile H2O
Quant-iT™ dsDNA HS Assay Kit, (Life Technologies catalog #: Q32851)
Oligonucleotide primers and adaptors
Low Elution Volume PCR purification Kit
Low Elution Volume Gel Extraction Kit
Materials for Agarose Gel Analysis
Magna Grip™ Rack 8 well (Cat.# 20-400) or similar magnetic rack.
Instrument capable of quantifying low concentrations of nucleic acids