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p70 S6 Kinase Assay Kit
Description:
p70 S6 Kinase Assay Kit
Trade Name:
Upstate (Millipore)
Qty/Pk:
1 kit
Key Applications:
Kinase Assay
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Entrez Gene Summary:
This gene encodes a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains 2 non-identical kinase catalytic domains and phosphorylates several residues of the S6 ribosomal protein. The kinase activity of this protein leads to an increase in protein synthesis and cell proliferation. Amplification of the region of DNA encoding this gene and overexpression of this kinase are seen in some breast cancer cell lines. Alternate translational start sites have been described and alternate transcriptional splice variants have been observed but have not been thoroughly characterized.
UniProt Summary:
FUNCTION: SwissProt: P23443 # Phosphorylates specifically ribosomal protein S6 in response to insulin or several classes of mitogens.
SIZE: 525 amino acids; 59140 Da
SUBUNIT: Interacts with PPP1R9A/neurabin-1 (By similarity).
SUBCELLULAR LOCATION: Cytoplasm (By similarity). Cell junction, synapse, synaptosome (By similarity).
TISSUE SPECIFICITY: Widely expressed.
SIMILARITY: SwissProt: P23443 ## Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. S6 kinase subfamily. & Contains 1 AGC-kinase C-terminal domain. & Contains 1 protein kinase domain.
SIZE: 525 amino acids; 59140 Da
SUBUNIT: Interacts with PPP1R9A/neurabin-1 (By similarity).
SUBCELLULAR LOCATION: Cytoplasm (By similarity). Cell junction, synapse, synaptosome (By similarity).
TISSUE SPECIFICITY: Widely expressed.
SIMILARITY: SwissProt: P23443 ## Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. S6 kinase subfamily. & Contains 1 AGC-kinase C-terminal domain. & Contains 1 protein kinase domain.
Quality Assurance:
Routinely evaluated by phosphorylation of S6 Kinase/Rsk2 Substrate Peptide 2.
Components:
- Assay Dilution Buffer I (ADBI) (Cat.# 20-108)
- S6 Kinase/Rsk2 Substrate Peptide 2 (Cat.# 12-243)
- P81 Phosphocellulose Squares (Cat.# 20-134)
- Inhibitor Cocktail (Cat.# 20-116)
- MgAc/ATP Cocktail (Cat.# 20-277)
Brand Family:
Upstate
UniProt Number:
Detection Methods:
- Radioactive
- Radioactive
Protein/Isoform Description:
S6 Kinase is a Ser/Thr kinase which phosphorylates the 40S ribosomal protein S6, and several translation-regulatory factors. S6 kinase is activated by phosphorylation of multiple sites by mTOR. S6K is an important regulator of protein synthesis, along with MAP Kinase. In neurons, S6K is linked to the cytoskeleton by neurabin, which may play a role in its activation. Rsk3 (MAPKAP Kinase 1c) and Rsk4 are also known as the Ribosomal Protein S6 Kinase, 90 kDa, polypeptide 2 and 6, respectively.
Gene Symbol:
- RPS6KB1
- STK14A
- S6Kalpha)
- S6K1
- p70-alpha
- p70S6k
- p70(S6K)-alpha
- PS6K
- p70-S6K
- p70
- P70S6K
- S6K
Product Name:
p70 S6 Kinase Assay Kit
Entrez Gene Number:
Analytes Available:
p70 S6