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MAP Kinase/Erk Immunoprecipitation Kinase Assay Kit, non-radioactive

Description:
MAP Kinase/Erk Immunoprecipitation Kinase Assay Kit, non-radioactive
Trade Name:
Upstate (Millipore)
Qty/Pk:
1 kit
Applications:
MAP Kinase/Erk Immunoprecipitation Kinase Assay Kit is designed to measure phosphotransferase activity in an immunocomplex formed between the MAP Kinase R2 antibody & MAP Kinase (p44mpk).
Key Applications:
Kinase Assay
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Entrez Gene Summary:
The protein encoded by this gene is a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. The activation of this kinase requires its phosphorylation by upstream kinases. Upon activation, this kinase translocates to the nucleus of the stimulated cells, where it phosphorylates nuclear targets. Two alternatively spliced transcript variants encoding the same protein, but differing in the UTRs, have been reported for this gene.
UniProt Summary:
FUNCTION: SwissProt: P28482 # Involved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK1. Phosphorylates EIF4EBP1; required for initiation of translation. Phosphorylates microtubule-associated protein 2 (MAP2). Phosphorylates SPZ1 (By similarity). Phosphorylates heat shock factor protein 4 (HSF4).
COFACTOR: Magnesium (By similarity).
SIZE: 360 amino acids; 41390 Da
SUBUNIT: Interacts with MORG1 (By similarity). Binds to HIV-1 Nef through its SH3 domain. This interaction inhibits its tyrosine- kinase activity. Interacts with HSF4.
DOMAIN: SwissProt: P28482 The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
PTM: Dually phosphorylated on Thr-185 and Tyr-187, which activates the enzyme.
SIMILARITY: Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily. & Contains 1 protein kinase domain.
Brand Family:
Upstate
Sales Restrictions:
This product is derived from bovine source. Export of this product to certain countries may be restricted. Please contact Customer Service or your local distributor to inquire about product availability and export options.
Gene Symbol:
  • MAPK1
  • p40
  • PRKM2
  • MAPK2
  • PRKM1
  • p42-MAPK
  • p38
  • p41
  • ERK
  • ERK-2
  • ERT1
  • P42MAPK
  • p41mapk
  • ERK2
Product Name:
MAP Kinase/Erk Immunoprecipitation Kinase Assay Kit, non-radioactive
Analytes Available:
MAP Kinase/Erk
Quality Assurance:
The assay kit is designed to measure phosphotransferase activity in an immunocomplex formed between the MAP Kinase R2 antibody and MAP Kinase (p44mpk). This precipitated enzyme is used to phosphorylate a specific substrate, myelin basic protein (MBP).
Components:
  • Assay Dilution Buffer, 5X (Cat.# 20-145)
  • Anti-MAP Kinase 1/2 (Erk1/2), agarose (Cat.# 16-111)
  • Anti-phospho-MBP, clone P12 (Cat.# 05-429)
  • MAP Kinase/Erk Substrate Cocktail II (Cat.# 20-166)
  • Inhibitor Cocktail (Cat.# 20-116)
  • Magnesium/ATP Cocktail (Cat.# 20-113)
UniProt Number:
Entrez Gene Number: