Ordering Information
- : 17-20000
- : 22 ChIP assays from tissue samples.
Product Images
300 µm of coronal mouse brain section with region specific microdissection of the hippocampus (A) and cerebral cortex (B). The isolated tissue was pl...
Magna ChIP™ G - Tissue Kit
Description:
Magna ChIP™ G Tissue Kit
Trade Name:
Upstate (Millipore)
Qty/Pk:
22 ChIP assays from tissue samples.
Product Overview:
Chromatin Immunoprecipitation (ChIP) is an important technique allowing the researcher to analyze in vivo interactions of proteins with genomic DNA. Any chromatin-associated or DNA binding protein can be analyzed with this technique, provided a good antibody to the protein exists. One can measure different proteins localized to a specific region of the genome, or the genome wide distribution of a specific protein. Another powerful application of this technique is to analyze changes in histone modifications that correlate with processes like transcription, mitosis or DNA repair.
Historically, ChIP protocols have been geared towards homogenous populations of cell culture. In contrast, tissue samples are more complex and the cells are heterogeneous thus making ChIP analysis less amenable. The Magna ChIP™ G Tissue Kit provides the tools necessary to obtain repeatable, reliable, and site specific tissue biopsies. Microdissected functionally related populations of cells within a heterogeneous tissue can thus be analyzed with ease, precision and certainty.
Historically, ChIP protocols have been geared towards homogenous populations of cell culture. In contrast, tissue samples are more complex and the cells are heterogeneous thus making ChIP analysis less amenable. The Magna ChIP™ G Tissue Kit provides the tools necessary to obtain repeatable, reliable, and site specific tissue biopsies. Microdissected functionally related populations of cells within a heterogeneous tissue can thus be analyzed with ease, precision and certainty.
Key Applications:
- Chromatin Immunoprecipitation (ChIP)
- Sample Prep
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Components:
- Magna ChIP G (2° to 8°C) - MAGNA0020
- Magna ChIP G (-20°C) - MAGNA0021
Brand Family:
Upstate
Presentation:
Two modules containing all necessary reagents to perform 22 individual chromatin immunoprecipitation (ChIP) reactions from tissue samples.
Storage Conditions:
Upon receipt, store components at the temperatures indicated on the labels. Kit components are stable for 6 months from date of shipment when used and stored as directed.
Product Name:
Magna ChIP™ G Tissue Kit
Materials Required but Not Delivered:
Cells, stimulated or treated as desired
Antibody for chromatin immunoprecipitation)
37% Formaldehyde
Taq DNA polymerase and dNTPs
Master Mix for qPCR
DNase and RNase-free sterile H2O
Quant-iT™ dsDNA HS Assay Kit, (Life Technologies catalog #: Q32851)
O.C.T. – Optimal Cutting Temperature compound
RNase A
Cryostat-microtome
Cryomold
Magna Grip™ Rack 8 well (Cat.# 20-400) or similar magnetic rack
Vortex mixer
Rotating wheel/platform
Microfuge
Sonicator
Thermomixer or Hybridization Oven
Variable temperature water bath or incubator
Variable volume (5-1000 l) pipettors + tips
Microfuge & PCR tubes
Thermal cycler
Filter-tip pipette tips
Antibody for chromatin immunoprecipitation)
37% Formaldehyde
Taq DNA polymerase and dNTPs
Master Mix for qPCR
DNase and RNase-free sterile H2O
Quant-iT™ dsDNA HS Assay Kit, (Life Technologies catalog #: Q32851)
O.C.T. – Optimal Cutting Temperature compound
RNase A
Cryostat-microtome
Cryomold
Magna Grip™ Rack 8 well (Cat.# 20-400) or similar magnetic rack
Vortex mixer
Rotating wheel/platform
Microfuge
Sonicator
Thermomixer or Hybridization Oven
Variable temperature water bath or incubator
Variable volume (5-1000 l) pipettors + tips
Microfuge & PCR tubes
Thermal cycler
Filter-tip pipette tips