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  • : 17-218
  • : 1 kit
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Affinity Precipitation/Immunoblot Analysis: 1 milligram of HeLa cell lysate was loaded with GDP (lane 1) or with GTPγS (lane 2) then incubated with Ra...

Ras Activation Assay Kit

Recommended Replacement for: SGT435
Description:
Ras Activation Assay Kit
Trade Name:
Upstate (Millipore)
Qty/Pk:
1 kit
Key Applications:
Enzyme Assay
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Entrez Gene Summary:
Members of the RAS superfamily of GTP-binding proteins, which includes MRAS, are membrane-anchored, intracellular signal transducers responsible for a variety of normal cellular functions. They are oncogenically activated in a significant fraction of tumors.[supplied by OMIM]
UniProt Summary:
FUNCTION: SwissProt: O14807 # May serve as an important signal transducer for a novel upstream stimuli in controlling cell proliferation. Weakly activates the MAP kinase pathway.
SIZE: 208 amino acids; 23846 Da
SUBUNIT: Interacts with RGL3 (By similarity).
SUBCELLULAR LOCATION: Cell membrane; Lipid-anchor; Cytoplasmic side (Potential).
TISSUE SPECIFICITY: Expression highly restricted to the brain and heart.
SIMILARITY: SwissProt: O14807 ## Belongs to the small GTPase superfamily. Ras family.
Brand Family:
Upstate
Promotional Text:
Special Offer on Antibodies! Click Here!
Replaces:
SGT435
Gene Symbol:
  • MRAS
  • M-RAs
  • M-RAS
  • FLJ42964
  • R-RAS3
  • RRAS3
Product Name:
Ras Activation Assay Kit
Quality Assurance:
This kit precipitated Ras-GTP from HeLa cell lysates that had been loaded with GTPγS. The precipitated Ras-GTP was detected by western immunoblot using 1μg/ml anti-Ras, clone RAS10
Components:
  • Ras Assay Reagent (Raf-1 RBD, agarose) (Cat.# 14-278)
  • Anti-Ras, clone RAS10 (Cat.# 05-516)
  • 100X GTPγS, 10mM (Cat.# 20-176)
  • 100X GDP, 100mM (Cat.# 20-177)
  • Mg2+ Lysis/Wash Buffer, 5X (Cat.# 20-168)
UniProt Number:
Packaging:
Kit capacity: 30 assays
Entrez Gene Number:

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