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Phospho-Serine/Threonine Assay Kit, 96-well, Chemiluminescence Detection
Description:
Phospho-Serine/Threonine Assay Kit, 96-well, Chemiluminescence Detection
Trade Name:
Upstate (Millipore)
Qty/Pk:
1 kit
Product Overview:
This kit is designed to measure the phosphotransferase activity of numerous serine/threonine kinases based on phosphorylation of a specific substrate, myelin basic protein (MBP). The phosphorylated substrate is detected using an antibody generated towards phosphorylated serine and threonine residues.
Key Applications:
Enzyme Assay
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Quality Assurance:
Routinely evaluated using MAPK2 (p42 Erk2) to phosphorylate MBP. Subsequent detection in western blot performed with Anti-phospho-Ser/Thr (mixed mouse monoclonal IgGs), Catalog # 44-006-MN.
Components:
- MBP Coated Microplate, 96 Well (Cat.# 30-011)
- MBP Coated Microplate, 96 Well (Cat.# 30-011)
- Kinase Assay Blocking Buffer (Cat.# 20-189)
- Kinase Assay Clearing Buffer (Cat.# 20-193)
- Chemi-Substrate A
- Chemi-Substrate B
- Anti-phospho-Ser/Thr
Brand Family:
Upstate
Detection Methods:
- Chemiluminescence
- Chemiluminescence
Modifications:
Phosphorylation
Protein/Isoform Description:
Approximately 400 of the 518 kinases in the human genome are serine/threonine specific protein kinases and approximately 40 serine/threonine specific protein phosphatases are encoded by the human genome. These enzymes are among the most studied biological molecules in both basic research as well as drug discovery laboratories. Given these kinases are involved in all aspects of cell signaling, it is not surprising to find that serine/threonine kinases are instrumental in many critical diseases including many cancers, diabetes and Alzheimer’s. As a result, many drug discovery programs are specifically targeting serine/threonine kinases in their research programs.
The new monoclonal anti-phosphoserine, clone 4A4 allows for the greatest detection of serine phosphorylation, irrespective of surrounding amino acids on a wide variety of substrates. 4A4 is proven to work with high affinity in a wide variety of applications including western blot, immunfluorescence, IHC, flow cytometry, ELISA and more. Additionally, in profiling studies, 4A4 detected more serine phosphorylated substrates in multiple cell lysates than any other anti-phosphoserine monoclonal antibody tested. As many substrates and kinases are phosphorylated on serine residues, this antibody has a very broad range of use.
The new monoclonal anti-phosphoserine, clone 4A4 allows for the greatest detection of serine phosphorylation, irrespective of surrounding amino acids on a wide variety of substrates. 4A4 is proven to work with high affinity in a wide variety of applications including western blot, immunfluorescence, IHC, flow cytometry, ELISA and more. Additionally, in profiling studies, 4A4 detected more serine phosphorylated substrates in multiple cell lysates than any other anti-phosphoserine monoclonal antibody tested. As many substrates and kinases are phosphorylated on serine residues, this antibody has a very broad range of use.
Product Name:
Phospho-Serine/Threonine Assay Kit, 96-well, Chemiluminescence Detection
Analytes Available:
Ser/Thr Kinases