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HAT Assay Kit
Description:
HAT Assay Kit
Trade Name:
Upstate (Millipore)
Qty/Pk:
1 kit
Key Applications:
ELISA
Application Notes:
Nuclear Extract (source of HAT enzyme activity): Prepare nuclear extracts using a modified protocol of Dignam, et al., Nucleic Acids Res. 22: 1475, 1983 (recommended, but other methods may be used). See citations for reference link. Use 1- 20μl per assay point in the HAT Reaction Cocktail. Optimal nuclear extract concentration will need to be determined empirically.
Species Reactivity:
All
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Entrez Gene Summary:
Histone acetylation, particularly of histone H4, plays an important role in replication-dependent chromatin assembly. This gene encodes a histone acetylase that contains A, B, and D motifs, present in many N-acetyltransferases, including those that acetylate substrates other than histones. Alternatively spliced transcript variants that encode different isoforms have been identified for this gene.
UniProt Summary:
FUNCTION: SwissProt: O14929 # May play a role in telomeric silencing. Acetylates soluble but not nucleosomal H4 at 'Lys-5' and 'Lys-12' and acetylates histone H2A at 'Lys-5'. HAT1 has intrinsic substrate specificity that modifies lysine in recognition sequence GXGKXG.
SIZE: 419 amino acids; 49513 Da
SUBUNIT: Heteromer of HAT1 and p46/HAT2 subunits.
SUBCELLULAR LOCATION: Nucleus. Cytoplasm. Note=Nuclear in S-phase cells and cytoplasmic.
SIMILARITY: SwissProt: O14929 ## Belongs to the HAT1 family.
SIZE: 419 amino acids; 49513 Da
SUBUNIT: Heteromer of HAT1 and p46/HAT2 subunits.
SUBCELLULAR LOCATION: Nucleus. Cytoplasm. Note=Nuclear in S-phase cells and cytoplasmic.
SIMILARITY: SwissProt: O14929 ## Belongs to the HAT1 family.
Quality Assurance:
Routinely evaluated in a HAT Assay. Biotinylated Histone H4 or Histone H3 peptides were acetylated with recombinant PCAF (14-309) in the presence of Acetyl-CoA and HAT Assay buffer.
Components:
- Streptavidin Coated Microwell Plate (Cat.# 20-183)
- Histone H3 Peptide, biotin conjugate (Cat.# 12-403)
- Histone H4 Peptide, biotin conjugate (Cat.# 12-405)
- HAT Assay Buffer, 5X (Cat.# 20-148)
- 07-129
- TBS, 20X (Cat.# 20-190)
- 10% BSA in TBS (Cat.# 20-191)
- Acetyl-Histone H3 (K9, K14) Peptide biotinylated; Histone H4 (Tetra Ac) Peptide biotinylated; Acetyl-CoA; Goat Anti-Rabbit IgG, HRP-conjugate; TMB (tetramethylbenzidine) Substrate Reagent A and TMB (tetramethylbenzidine) Substrate Reagent B
Brand Family:
Upstate
UniProt Number:
Host:
Rabbit
Detection Methods:
Fluorescent
Packaging:
Kit capacity: 96 assays
Protein/Isoform Description:
HAT (Histone acetyltransferases) are a family of enzymes that catalyze the addition of an acetyl group to the epsilon-amino group of an amino-terminal lysine residue of one of the core histone proteins (H2A, H2B, H3 and H4). Non-histone substrates (p53, nuclear receptors) have also been reported for some of the HATs. Histone acetyltransferases are separated into two types: type A HATs (PCAF, P300, GCN5, MOZ) are located in the nucleus, and many play important roles in the regulation of gene expression by functioning as transcriptional co-activators. Type B HATs (HAT1) are located in the cytoplasm and are intimately involved with chromatin synthesis and assembly of nascent histones into chromosomes.
Gene Symbol:
HAT1
Product Name:
HAT Assay Kit
Entrez Gene Number: