Anti-Cdc42 Immunoblotting Kit

Anti-Cdc42 Immunoblotting Kit

Upstate (Millipore)

1 kit

Anti-cdc42 is a mouse monoclonal IgG1 that recognizes cdc42 from human, mouse, rat and dog

Western Blotting

Human

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The protein encoded by this gene is a small GTPase of the Rho-subfamily, which regulates signaling pathways that control diverse cellular functions including cell morphology, migration, endocytosis and cell cycle progression. This protein is highly similar to Saccharomyces cerevisiae Cdc 42, and is able to complement the yeast cdc42-1 mutant. The product of oncogene Dbl was reported to specifically catalyze the dissociation of GDP from this protein. This protein could regulate actin polymerization through its direct binding to Neural Wiskott-Aldrich syndrome protein (N-WASP), which subsequently activates Arp2/3 complex. Alternative splicing of this gene results in multiple transcript variants.

FUNCTION: SwissProt: P60953 # Plasma membrane-associated small GTPase which cycles between an active GTP-bound and an inactive GDP-bound state. In active state binds to a variety of effector proteins to regulate cellular responses. Involved in epithelial cell polarization processes. Causes the formation of thin, actin-rich surface projections called filopodia.
SIZE: 191 amino acids; 21311 Da
SUBUNIT: The GTP-bound form interacts with CCPG1 (By similarity). Interacts with CDC42EP1, CDC42EP2, CDC42EP3, CDC42EP4, CDC42EP5, PARD6A, PARD6B and PARD6G in a GTP-dependent manner. Interacts with activated CSPG4 and with BAIAP2. Interacts with Zizimin1/DOCK9 and Zizimin2/DOCK11, which activate it by exchanging GDP for GTP. Interacts with NET1 and TCGAP/SNX26. Part of a complex with PARD3, PARD6A or PARD6B and PRKCI or PRKCZ.
SUBCELLULAR LOCATION: Cell membrane; Lipid-anchor; Cytoplasmic side (Potential).
SIMILARITY: SwissProt: P60953 ## Belongs to the small GTPase superfamily. Rho family. CDC42 subfamily.

Routinely evaluated by immunoblot on RIPA lysates from HL-60 cells.

• Anti-cdc42, (mouse monoclonal IgG1), Catalog # 05-542
• Immunoblot Blocking Reagent (Cat.# 20-200)
• Goat Anti-Mouse IgG, HRP conjugate (Cat.# 12-349)

Upstate

See Certificate of Analysis for details

P60953

Mouse

Stable for 1 year at -20°C from date of shipment.
Note: Catalog # 12-349, Goat Anti-Mouse IgG, HRP-conjugate is light sensitive, handle accordingly.

Kit capacity: 10 immunoblots per kit

cdc42 is a member of the Rho family of small GTP-binding proteins, and plays roles in cytoskeletal Actin organization as well as transformation. Rac is activated downstream of cdc42, or independently by Ras signaling, and in turn Rac stimulates Rho. Activation of cdc42 stimulates filopodia formation, whereas Rac results in the formation of lamellipodia, and Rho stimulates formation of actin stress fibers. Effectors of the cdc42/Rac pathway include NADPH Oxidase (Phox) and the SAPK1/JNK family of kinases. The latter pathway is activated by the PAK kinases, which are direct targets of cdc42.

• CDC42
• G25K
• CDC42Hs

Anti-Cdc42 Immunoblotting Kit

• NM_001039802.1
• NM_001791.3
• NM_044472.2