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WB: 10μg Control Histones, untreated (lane1) or colcemid-treated (lane 2), probed with anti-phospho-Histone H3, Ser10 (#06-570).
Control Histones (+/- colcemid)
Description:
Control Histones (+/- colcemid)
Trade Name:
Upstate (Millipore)
Product Overview:
Product Description: Core histones, including histone H1, purified by acid extraction precipitation from log phase untreated and colcemid-treated HeLa cells.
Key Applications:
Western Blotting
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Species:
Human
Quality Assurance:
Routinely evaluated by western blot. Anti-phospho-Histone H3 (Ser10) (06-570) or Anti-phospho-Histone H3 (Ser28) (07-145) were tested on histones from untreated and colcemid-treated HeLa cells.
Components:
- Control Histones, untreated
- Control Histones, treated
Storage Conditions:
Lyophilized: Stable for 2 years at -20°C from date of shipment, when stored with provided desiccant.
Reconstituted: Stable for 6 months at -70°C.
Rehydration:
Aseptically reconstitute to 1 mg/mL with sterile water. Control Histones, untreated may be rehydrated in 2X RSB (reticulocyte standard buffer) if used exclusively for immunoblotting. Aliquot to avoid repeated freezing and thawing.
RSB, 1X: 10 mM Tris-HCl, pH 7.6, 3 mM MgCl2, 10 mM NaCl, 1 mM PMSF, and protein phosphatase inhibitors
Reconstituted: Stable for 6 months at -70°C.
Rehydration:
Aseptically reconstitute to 1 mg/mL with sterile water. Control Histones, untreated may be rehydrated in 2X RSB (reticulocyte standard buffer) if used exclusively for immunoblotting. Aliquot to avoid repeated freezing and thawing.
RSB, 1X: 10 mM Tris-HCl, pH 7.6, 3 mM MgCl2, 10 mM NaCl, 1 mM PMSF, and protein phosphatase inhibitors
Packaging:
Kit capacity: 50 assays
Gene Symbol:
- HIST1H1E
- H1F4
- H1.4
- dJ221C16.5
- MGC116819
Protein or Enzyme Type:
Histones and Histone-Modifying Enzymes