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WB: 10μg Control Histones, untreated (lane1) or colcemid-treated (lane 2), probed with anti-phospho-Histone H3, Ser10 (#06-570).
Control Histones (+/- colcemid)
Description:
Control Histones (+/- colcemid)
Trade Name:
Upstate (Millipore)
Qty/Pk:
1 kit
Product Overview:
Product Description: Core histones, including histone H1, purified by acid extraction precipitation from log phase untreated and colcemid-treated HeLa cells.
Key Applications:
Western Blotting
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Entrez Gene Summary:
Histones are basic nuclear proteins responsible for nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a member of the histone H1 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6.
UniProt Summary:
FUNCTION: SwissProt: P10412 # Histones H1 are necessary for the condensation of nucleosome chains into higher order structures.
SIZE: 219 amino acids; 21865 Da
SUBCELLULAR LOCATION: Nucleus.
SIMILARITY: SwissProt: P10412 ## Belongs to the histone H1/H5 family.
MISCELLANEOUS: This variant accounts for 60% of histone H1.
SIZE: 219 amino acids; 21865 Da
SUBCELLULAR LOCATION: Nucleus.
SIMILARITY: SwissProt: P10412 ## Belongs to the histone H1/H5 family.
MISCELLANEOUS: This variant accounts for 60% of histone H1.
Species:
Human
Quality Assurance:
Routinely evaluated by western blot. Anti-phospho-Histone H3 (Ser10) (06-570) or Anti-phospho-Histone H3 (Ser28) (07-145) were tested on histones from untreated and colcemid-treated HeLa cells.
Components:
- Control Histones, untreated
- Control Histones, treated
Brand Family:
Upstate
UniProt Number:
Storage Conditions:
Lyophilized: Stable for 2 years at -20°C from date of shipment, when stored with provided desiccant.
Reconstituted: Stable for 6 months at -70°C.
Rehydration:
Aseptically reconstitute to 1 mg/mL with sterile water. Control Histones, untreated may be rehydrated in 2X RSB (reticulocyte standard buffer) if used exclusively for immunoblotting. Aliquot to avoid repeated freezing and thawing.
RSB, 1X: 10 mM Tris-HCl, pH 7.6, 3 mM MgCl2, 10 mM NaCl, 1 mM PMSF, and protein phosphatase inhibitors
Reconstituted: Stable for 6 months at -70°C.
Rehydration:
Aseptically reconstitute to 1 mg/mL with sterile water. Control Histones, untreated may be rehydrated in 2X RSB (reticulocyte standard buffer) if used exclusively for immunoblotting. Aliquot to avoid repeated freezing and thawing.
RSB, 1X: 10 mM Tris-HCl, pH 7.6, 3 mM MgCl2, 10 mM NaCl, 1 mM PMSF, and protein phosphatase inhibitors
Packaging:
Kit capacity: 50 assays
Gene Symbol:
- HIST1H1E
- H1F4
- H1.4
- dJ221C16.5
- MGC116819
Protein or Enzyme Type:
Histones and Histone-Modifying Enzymes
Product Name:
Control Histones (+/- colcemid)
Entrez Gene Number: