PP2A Immunoprecipitation Phosphatase Assay Kit

PP2A Immunoprecipitation Phosphatase Assay Kit

Upstate (Millipore)

25 assays

Phosphatase Assay

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The protein encoded by this gene is one of the three catalytic subunits of protein phosphatase 1 (PP1). PP1 is a serine/threonine specific protein phosphatase known to be involved in the regulation of a variety of cellular processes, such as cell division, glycogen metabolism, muscle contractility, protein synthesis, and HIV-1 viral transcription. Increased PP1 activity has been observed in the end stage of heart failure. Studies in both human and mice suggest that PP1 is an important regulator of cardiac function. Mouse studies also suggest that PP1 functions as a suppressor of learning and memory. Three alternatively spliced transcript variants encoding different isoforms have been found for this gene.

FUNCTION: SwissProt: P67775 # PP2A can modulate the activity of phosphorylase B kinase casein kinase 2, mitogen-stimulated S6 kinase, and MAP-2 kinase. Can dephosphorylate SV40 large T antigen and p53. Dephosphorylates SV40 large T antigen, preferentially on serine residues 120, 123, 677, and perhaps 679. The C subunit was most active, followed by the AC form, which was more active than the ABC form, and activity of all three forms was strongly stimulated by manganese, and to a lesser extent by magnesium. Dephosphorylation by the AC form, but not C or ABC form is inhibited by small T antigen.
COFACTOR: Binds 1 iron ion per subunit (By similarity). & Binds 1 manganese ion per subunit (By similarity).
SIZE: 309 amino acids; 35594 Da
SUBUNIT: PP2A consists of a common heterodimeric core enzyme, composed of a 36 kDa catalytic subunit (subunit C) and a 65 kDa constant regulatory subunit (PR65 or subunit A), that associates with a variety of regulatory subunits. Proteins that associate with the core dimer include three families of regulatory subunits B (the R2/B/PR55/B55, R3/B''/PR72/PR130/PR59 and R5/B'/B56 families), the 48 kDa variable regulatory subunit, viral proteins, and cell signaling molecules.
SUBCELLULAR LOCATION: Cytoplasm.
PTM: Reversibly methyl esterified on Leu-309. Carboxyl methylation may play a role in holoenzyme assembly. It varies during the cell cycle. & Phosphorylation of either threonine (by autophosphorylation- activated protein kinase) or tyrosine results in inactivation of the phosphatase. Auto-dephosphorylation has been suggested as a mechanism for reactivation.
SIMILARITY: SwissProt: P67775 ## Belongs to the PPP phosphatase family. PP-1 subfamily.

Routinely evaluated by immunoprecipitation of purified PP2A (14-111) or PP2A from a cellular lysate. The activity is assessed by dephosphorylation of the phosphopeptide (K-R-pT-I-R-R).

• Malachite Green Solution A (Cat.# 20-105)
• Malachite Green Additive (Cat.# 20-104)
• Phosphate Standard (Cat.# 20-103)
• Threonine Phosphopeptide (K-R-pT-I-R-R) (Cat.# 12-219)
• Anti-PP2A, C subunit, clone 1D6 (Cat.# 05-421)
• pNPP Ser/Thr Assay Buffer (Cat.# 20-179)
• Protein A Agarose
• 96-Well Microtiter Plate

Upstate

P67775

Kit capacity: 25 assays

• PPP2CA
• PP2CA
• PP2A-alpha
• PP2Ac
• RP-C

PP2A Immunoprecipitation Phosphatase Assay Kit

NP_002706

PP2A