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Affinity Precipitation and WB: Following treatment for affinity precipitation, HEK293 lysate in TLB preincubated with GDP, then precipitated with Ral...
Rap1 Activation Assay Kit
Description:
Rap1 Activation Assay Kit
Trade Name:
Upstate (Millipore)
Qty/Pk:
1 kit
Key Applications:
Affinity Binding Assay
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
UniProt Summary:
FUNCTION: SwissProt: Q9NYB0 # May play a role in telomere length regulation.
SIZE: 399 amino acids; 44260 Da
SUBUNIT: Homodimer. Binds to TRF2 (but not TRF1) with its C- terminus.
SUBCELLULAR LOCATION: Nucleus. Note=Colocalizes with telomeric DNA in interphase and metaphase cells.
TISSUE SPECIFICITY: Ubiquitous. Highly expressed.
PTM: Phosphorylated upon DNA damage, probably by ATM or ATR.
SIMILARITY: SwissProt: Q9NYB0 ## Contains 1 BRCT domain. & Contains 1 Myb-like domain.
MISCELLANEOUS: Recruited to telomeres by TRF2; seemingly it does not directly bind to DNA itself.
SIZE: 399 amino acids; 44260 Da
SUBUNIT: Homodimer. Binds to TRF2 (but not TRF1) with its C- terminus.
SUBCELLULAR LOCATION: Nucleus. Note=Colocalizes with telomeric DNA in interphase and metaphase cells.
TISSUE SPECIFICITY: Ubiquitous. Highly expressed.
PTM: Phosphorylated upon DNA damage, probably by ATM or ATR.
SIMILARITY: SwissProt: Q9NYB0 ## Contains 1 BRCT domain. & Contains 1 Myb-like domain.
MISCELLANEOUS: Recruited to telomeres by TRF2; seemingly it does not directly bind to DNA itself.
Quality Assurance:
Routinely evaluated by affinity precipitation assay. Ral GDS RBD, agarose precipitated GTP-Rap1 from HEK293 lysates. The precipitated GTP-Rap1 was detected by immunoblot analysis using Anti-Rap1.
Components:
- 100X GTPγS, 10mM (Cat.# 20-176)
- 100X GDP, 100mM (Cat.# 20-177)
- Rap1 Activation Lysis Buffer, 2X
- Anti-Rap1
- Rap1 Assay Reagent (Ral GDS-RBD, agarose) (Cat.# 14-455)
Brand Family:
Upstate
UniProt Number:
Packaging:
Kit capacity: 20-30 assays
Protein/Isoform Description:
Rap1 is a member of the Ras-family of small G-Proteins. It was initially thought to be a Ras antagonist that induces T-cell anergy. Rap1 has also been shown to promote B-cell adhesion and migration toward chemokines.
Gene Symbol:
- TERF2IP
- hRap1
- DRIP5
- RAP1
Product Name:
Rap1 Activation Assay Kit
Entrez Gene Number: