Herstatin Detection Kit (ELISA based)

Herstatin Detection Kit (ELISA based)

Upstate (Millipore)

1 kit

The herstatin detection assay is a sandwich enzyme linked immunosorbent assay (ELISA) employing two mouse monoclonal antibodies specific for herstatin. Strip-wells are coated with the capture antibody and samples are incubated, allowing binding of herstatin to the capture antibody. Unbound material is removed in the wash steps and bound herstatin is detected using the biotinylated reporter antibody. Horseradish peroxidase (HRP)-conjugated streptavidin is then bound to the reporter antibody and Tetramethylbenzidine (TMB) is provided as the HRP substrate for colorimetric detection.

ELISA

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This gene encodes a member of the epidermal growth factor (EGF) receptor family of receptor tyrosine kinases. This protein has no ligand binding domain of its own and therefore cannot bind growth factors. However, it does bind tightly to other ligand-bound EGF receptor family members to form a heterodimer, stabilizing ligand binding and enhancing kinase-mediated activation of downstream signalling pathways, such as those involving mitogen-activated protein kinase and phosphatidylinositol-3 kinase. Allelic variations at amino acid positions 654 and 655 of isoform a (positions 624 and 625 of isoform b) have been reported, with the most common allele, Ile654/Ile655, shown here. Amplification and/or overexpression of this gene has been reported in numerous cancers, including breast and ovarian tumors. Alternative splicing results in several additional transcript variants, some encoding different isoforms and others that have not been fully characterized.

FUNCTION: SwissProt: P04626 # Essential component of a neuregulin-receptor complex, although neuregulins do not interact with it alone. GP30 is a potential ligand for this receptor. Not activated by EGF, TGF- alpha and amphiregulin.
SIZE: 1255 amino acids; 137910 Da
SUBUNIT: Heterodimer with each of the other ERBB receptors (Potential). Interacts with PRKCABP and PLXNB1. Part of a complex with EGFR and either PIK3C2A or PIK3C2B. May interact with PIK3C2B when phosphorylated on Tyr-1196. Interacts with MEMO when phosphorylated on Tyr-1248.
SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
PTM: Ligand-binding increases phosphorylation on tyrosine residues (By similarity).
SIMILARITY: SwissProt: P04626 ## Belongs to the protein kinase superfamily. Tyr protein kinase family. EGF receptor subfamily. & Contains 1 protein kinase domain.

Routinely evaluated by ELISA

• Herstatin Reporter Antibody, biotin conjugate
• Herstatin Capture Antibody
• 20X TBS, 2% Tween-20
• High Binding 96-well Strip Plate
• Herstatin Standard and Serum Diluent
• Streptavidin HRP
• 10% BSA in TBS
• TMB Substrate Reagent A & B

Upstate

This product is not available for sale in the state of Connecticut.

P04626

Kit capacity: 96 assays

Herstatin is an autoinhibitor of the ErbB family consisting of subdomains I and II of the HER-2 extracellular domain and a novel c-terminal domain encoded by a HER-2 intron. Herstatin binds to HER-2 and to the EGF Receptor, blocking receptor oligomerization and tyrosine phosphorylation.

• ERBB2
• HER2
• HER-2
• NGL
• herstatin
• erbB-2
• C-erbB-2
• NEU
• TKR1
• p185erbB2
• HER-2/neu

Herstatin Detection Kit (ELISA based)

• NM_001005862.1
• NM_004448.2