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B-Raf Kinase Cascade Assay Kit
Description:
B-Raf Kinase Cascade Assay Kit
Trade Name:
Upstate (Millipore)
Qty/Pk:
1 kit
Product Overview:
The assay kit is designed to measure B-Raf dependent phosphotransferase activity in a kinase cascade reaction using recombinant MEK1, unactive as a B-Raf substrate. Recombinant MAPK2/Erk2, unactive is phosphorylated and activated by the activated MEK1 leading to phosphorylation of a MAP Kinase substrate, myelin basic protein (MBP). [γ-32P]ATP is the final phosphate donor.
Key Applications:
- IP-Kinase Assay
- Kinase Assay
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
UniProt Summary:
FUNCTION: SwissProt: P15056 # Involved in the transduction of mitogenic signals from the cell membrane to the nucleus. May play a role in the postsynaptic responses of hippocampal neuron.
COFACTOR: Binds 2 zinc ions per subunit (By similarity).
SIZE: 766 amino acids; 84437 Da
SUBUNIT: Interacts with RIT1 (By similarity).
SUBCELLULAR LOCATION: Cytoplasm.
TISSUE SPECIFICITY: Brain and testis.
DISEASE: SwissProt: P15056 # Defects in BRAF are a cause of cardiofaciocutaneous syndrome (CFC syndrome) [MIM:115150]; also known as cardio-facio- cutaneous syndrome. CFC syndrome is characterized by a distinctive facial appearance, heart defects and mental retardation. Heart defects include pulmonic stenosis, atrial septal defects and hypertrophic cardiomyopathy. Some affected individuals present with ectodermal abnormalities such as sparse, friable hair, hyperkeratotic skin lesions and a generalized ichthyosis-like condition. Typical facial features are similar to Noonan syndrome. They include high forehead with bitemporal constriction, hypoplastic supraorbital ridges, downslanting palpebral fissures, a depressed nasal bridge, and posteriorly angulated ears with prominent helices. The inheritance of CFC syndrome is autosomal dominant. & Defects in BRAF are involved in a wide range of cancers. & Defects in BRAF are involved in lung cancer [MIM:211980]. & Defects in BRAF are involved in non-Hodgkin lymphoma (NHL) [MIM:605027]. NHL is a cancer that starts in cells of the lymph system, which is part of the body's immune system. NHLs can occur at any age and are often marked by enlarged lymph nodes, fever and weight loss. & Defects in BRAF may be a cause of colorectal cancer (CRC) [MIM:114500].
SIMILARITY: SwissProt: P15056 ## Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. RAF subfamily. & Contains 1 phorbol-ester/DAG-type zinc finger. & Contains 1 protein kinase domain. & Contains 1 RBD (Ras-binding) domain.
COFACTOR: Binds 2 zinc ions per subunit (By similarity).
SIZE: 766 amino acids; 84437 Da
SUBUNIT: Interacts with RIT1 (By similarity).
SUBCELLULAR LOCATION: Cytoplasm.
TISSUE SPECIFICITY: Brain and testis.
DISEASE: SwissProt: P15056 # Defects in BRAF are a cause of cardiofaciocutaneous syndrome (CFC syndrome) [MIM:115150]; also known as cardio-facio- cutaneous syndrome. CFC syndrome is characterized by a distinctive facial appearance, heart defects and mental retardation. Heart defects include pulmonic stenosis, atrial septal defects and hypertrophic cardiomyopathy. Some affected individuals present with ectodermal abnormalities such as sparse, friable hair, hyperkeratotic skin lesions and a generalized ichthyosis-like condition. Typical facial features are similar to Noonan syndrome. They include high forehead with bitemporal constriction, hypoplastic supraorbital ridges, downslanting palpebral fissures, a depressed nasal bridge, and posteriorly angulated ears with prominent helices. The inheritance of CFC syndrome is autosomal dominant. & Defects in BRAF are involved in a wide range of cancers. & Defects in BRAF are involved in lung cancer [MIM:211980]. & Defects in BRAF are involved in non-Hodgkin lymphoma (NHL) [MIM:605027]. NHL is a cancer that starts in cells of the lymph system, which is part of the body's immune system. NHLs can occur at any age and are often marked by enlarged lymph nodes, fever and weight loss. & Defects in BRAF may be a cause of colorectal cancer (CRC) [MIM:114500].
SIMILARITY: SwissProt: P15056 ## Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. RAF subfamily. & Contains 1 phorbol-ester/DAG-type zinc finger. & Contains 1 protein kinase domain. & Contains 1 RBD (Ras-binding) domain.
Quality Assurance:
Routinely evalauted by Kinase Assay
Components:
- B-Raf (δ1-415), active (Cat.# 14-530)
- MEK1, unactive, rabbit (Cat.# 14-205)
- MAP Kinase 2/Erk2, unactive (Cat.# 14-198)
- Assay Dilution Buffer I (ADBI) (Cat.# 20-108)
- Magnesium/ATP Cocktail (Cat.# 20-113)
- P81 Phosphocellulose Squares (Cat.# 20-134)
- Myelin Basic Protein
Brand Family:
Upstate
Sales Restrictions:
This product is derived from bovine source. Export of this product to certain countries may be restricted. Please contact Customer Service or your local distributor to inquire about product availability and export options.
UniProt Number:
Packaging:
Kit capacity: 50 kinase assays per kit
Gene Symbol:
- BRAF
- B-raf-1
- BRAF1
- MGC126806
- B-Raf
- p94
- MGC138284
- RAFB1
Product Name:
B-Raf Kinase Cascade Assay Kit
Entrez Gene Number:
Analytes Available:
B-Raf