B-Raf Kinase Cascade Assay Kit
Description:
B-Raf Kinase Cascade Assay Kit
Trade Name:
Upstate (Millipore)
Product Overview:
The assay kit is designed to measure B-Raf dependent phosphotransferase activity in a kinase cascade reaction using recombinant MEK1, unactive as a B-Raf substrate. Recombinant MAPK2/Erk2, unactive is phosphorylated and activated by the activated MEK1 leading to phosphorylation of a MAP Kinase substrate, myelin basic protein (MBP). [γ-32P]ATP is the final phosphate donor.
Key Applications:
- IP-Kinase Assay
- Kinase Assay
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Quality Assurance:
Routinely evalauted by Kinase Assay
Kit or Assay Type:
Kinase & Phosphatase Assays
Components:
- B-Raf (δ1-415), active (Cat.# 14-530)
- MEK1, unactive, rabbit (Cat.# 14-205)
- MAP Kinase 2/Erk2, unactive (Cat.# 14-198)
- Assay Dilution Buffer I (ADBI) (Cat.# 20-108)
- Magnesium/ATP Cocktail (Cat.# 20-113)
- P81 Phosphocellulose Squares (Cat.# 20-134)
- Myelin Basic Protein
Sales Restrictions:
This product is derived from bovine source. Export of this product to certain countries may be restricted. Please contact Customer Service or your local distributor to inquire about product availability and export options.
Packaging:
Kit capacity: 50 kinase assays per kit
Gene Symbol:
- BRAF
- B-raf-1
- BRAF1
- MGC126806
- B-Raf
- p94
- MGC138284
- RAFB1
Analytes Available:
B-Raf