B-Raf Kinase Assay Kit, Chemiluminescence Detection
Description:
B-Raf Kinase Assay Kit, Chemiluminescence Detection
Trade Name:
Upstate (Millipore)
Product Overview:
Intended for the assay of recombinant B-Raf (supplied) kinase activity with experimental variables as determined by the user. For assay of B-Raf activity from a cell lysate, we recommend an immunoprecipitation-kinase assay, using anti-B-Raf antibody (07-453).
Key Applications:
- Kinase Assay
- Western Blotting
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Quality Assurance:
phosphorylation of MEK1
Kit or Assay Type:
Kinase & Phosphatase Assays
Components:
- B-Raf (δ1-415), active (Cat.# 14-530)
- MEK1, unactive (Cat.# 14-420)
- Assay Dilution Buffer I (ADBI) (Cat.# 20-108)
- Magnesium/ATP Cocktail (Cat.# 20-113)
- Anti-phospho-MEK1 (Ser218/222)/MEK2 (Ser222/226) (Cat.# 07-461)
Detection Methods:
- Chemiluminescence
- Chemiluminescence
Packaging:
Kit capacity: 50 assays
Gene Symbol:
- BRAF
- B-raf-1
- BRAF1
- MGC126806
- B-Raf
- p94
- MGC138284
- RAFB1
Analytes Available:
B-Raf