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Caspase 3/7 Assay Kit (Ac-DEVD-AMC Substrate)
Description:
Caspase 3/7 Assay Kit (Ac-DEVD-AMC Substrate)
Trade Name:
Upstate (Millipore)
Qty/Pk:
1 kit
Product Overview:
Caspase 3/7 activity is measured from the release of a fluorophore (AMC) from the substrate Ac-DEVD-AMC.
Key Applications:
Activity Assay
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Entrez Gene Summary:
This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. The precursor of this caspase is cleaved by caspase 3 and 10. It is activated upon cell death stimuli and induces apoptosis. Alternative splicing results in four transcript variants, encoding three distinct isoforms.
UniProt Summary:
FUNCTION: SwissProt: P55210 # Involved in the activation cascade of caspases responsible for apoptosis execution. Cleaves and activates sterol regulatory element binding proteins (SREBPs). Proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp- -Gly- 217' bond. Overexpression promotes programmed cell death.
SIZE: 303 amino acids; 34277 Da
SUBUNIT: Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 20 kDa (p20) and a 11 kDa (p11) subunit.
SUBCELLULAR LOCATION: Cytoplasm.
TISSUE SPECIFICITY: Highly expressed in lung, skeletal muscle, liver, kidney, spleen and heart, and moderately in testis. No expression in the brain.
PTM: Cleavages by granzyme B or caspase-10 generate the two active subunits. Propeptide domains can also be cleaved efficiently by caspase-3. Active heterodimers between the small subunit of caspase-7 and the large subunit of caspase-3, and vice versa, also occur.
SIMILARITY: SwissProt: P55210 ## Belongs to the peptidase C14 family.
SIZE: 303 amino acids; 34277 Da
SUBUNIT: Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 20 kDa (p20) and a 11 kDa (p11) subunit.
SUBCELLULAR LOCATION: Cytoplasm.
TISSUE SPECIFICITY: Highly expressed in lung, skeletal muscle, liver, kidney, spleen and heart, and moderately in testis. No expression in the brain.
PTM: Cleavages by granzyme B or caspase-10 generate the two active subunits. Propeptide domains can also be cleaved efficiently by caspase-3. Active heterodimers between the small subunit of caspase-7 and the large subunit of caspase-3, and vice versa, also occur.
SIMILARITY: SwissProt: P55210 ## Belongs to the peptidase C14 family.
Quality Assurance:
routinely evaluated by the inhibition of Ac-DEVD-CHO by Caspase 3
Components:
- 2X Caspase Buffer
- Dithiothreitol
- 2 White flat bottom 96-well plates
- Caspase Substrate
- Caspase 3, active
Brand Family:
Upstate
UniProt Number:
Detection Methods:
Fluorescent
Packaging:
Kit capacity: 192 assays per kit
Gene Symbol:
- CASP3
- apopain
- CASP-3
- procaspase3
- CPP32B
- SCA-1
- CPP-32
- Apopain
- CPP32
- Yama
Product Name:
Caspase 3/7 Assay Kit (Ac-DEVD-AMC Substrate)
Entrez Gene Number: