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  • : 17-371
  • : 22 assays

EZ-ChIP™ - Chromatin Immunoprecipitation Kit

Description:
EZ-ChIP™
Trade Name:
  • EZ-ChIP
  • Upstate (Millipore)
Qty/Pk:
22 assays
Product Overview:
Chromatin Immunoprecipitation (ChIP) is an important technique allowing the researcher to analyze in vivo interactions of proteins with genomic DNA. Any chromatin-associated or DNA binding protein can be analyzed with this technique, provided a good antibody to the protein exists. One can measure different proteins localized to a specific region of the genome, or the genome wide distribution of a specific protein. Another powerful application of this technique is to analyze changes in histone modifications that correlate with processes like transcription, mitosis or DNA repair.

Features & Benefits:
Easier: Spin columns make DNA purification easier and more reliable - no more messy phenol-chloroform extractions.
Quicker: All reagents to process your samples are included - you don't have to spend valuable time making them.
Greater Reproducibility: Positive and negative control antibodies and PCR primers are included to help validate your results and to troubleshoot your experiments.
Applications:
Contains all necessary reagents to perform 22 individual chromatin immunoprecipitation (ChIP) reactions.
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Components:
  • DNA Purification Spin Columns and Collection Tubes
  • ChIP Blocked Protein G Agarose
  • Anti-RNA Polymerase II
  • Control PCR Primers
  • Normal Mouse IgG
  • Bind, Wash and Elution Reagents
  • Protease Inhibitor Cocktail II
  • RNase A
  • Proteinase K
  • All required buffers
  • ChIP Dilution Buffer
  • Low Salt Immune Complex Wash Buffer
  • High Salt Immune Complex Wash Buffer
  • LiCl Immune Complex Wash Buffer
  • TE Buffer
  • 0.5M EDTA
  • 5M NaCl
  • SDS Lysis Buffer
  • 1M Tris-HCl, pH 6.5
  • 10X PBS
  • 10X Glycine
  • 1M NaHCO3
  • Control Primers
  • 20% SDS
  • Spin Filters
  • Collection Tubes
  • Bind Reagent A
  • Wash Reagent B
  • Elution Reagent C
Brand Family:
Upstate
Presentation:
Contains all necessary reagents to perform 22 individual chromatin immunoprecipitation (ChIP) reactions. Supplied buffers are sufficient to generate chromatin from up to five 15 cm plates of cultured cells, each plate providing up to 10 chromatin preparations (varies with cell and assay type).
Storage Conditions:
Upon receipt, store components at the temperatures indicated on the labels. Kit components are stable for 1 year from date of shipment when stored as directed.
Packaging:
Kit capacity: 22 chromatin immunoprecipitation assays
Product Name:
EZ-ChIP™

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