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The provided cGMP standard containing 0.5 mM cGMP (500,000 pmol/ml) was used in this experiment. Eight serial dilutions were prepared in Lysis buffer. ...
cGMP Assay overview
cGMP HTS Immunoassay Kit (Chemiluminescent)
Description:
cGMP HTS Immunoassay Kit (Chemiluminescent)
Product Overview:
Upstate®'s cGMP HTS Immunoassay kit is a competitive immunoassay for highly sensitive and rapid chemiluminescent quantitation of cyclic GMP (cGMP, Guanosine 3', 5'-cyclic monophosphate) from extracts, lysates or supernatants of cells of any species. The kit comprises a specific anti-cGMP antibody which recognizes all species, an alkaline phosphatase (AP)-labeled cGMP conjugate, two 96 well microplates pre-coated with ananti-Rabbit antibody, cGMP standard, and an Alkaline Phosphate chemiluminescent substrate. This kit contains antibody coated plates and all reagents required to perform 192 assays.
Key Applications:
ELISA
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Alternate Names:
cyclic GMP ELISA
Sensitivity:
Cross-Reactivity
cGMP 100%,
cAMP <0.1%,
GTP <0.01%,
GMP <0.01%,
CTP <0.01%,
AMP <0.01%,
ADP <0.01%,
ATP <0.01%.
cGMP 100%,
cAMP <0.1%,
GTP <0.01%,
GMP <0.01%,
CTP <0.01%,
AMP <0.01%,
ADP <0.01%,
ATP <0.01%.
Materials Required but Not Delivered:
1. Multi-channel or repeating pipettes
2. Pipettors & tips capable of accurately measuring 10-1000 µL
3. Plate shaker (optional)
4. Graduated serological pipettes
5. Luminometer capable of reading 96-well microplates.
6. Polystyrene test tubes for standard and sample dilutions
7. Mechanical vortex
8. Containers for dilutions
2. Pipettors & tips capable of accurately measuring 10-1000 µL
3. Plate shaker (optional)
4. Graduated serological pipettes
5. Luminometer capable of reading 96-well microplates.
6. Polystyrene test tubes for standard and sample dilutions
7. Mechanical vortex
8. Containers for dilutions
Components:
- 1. White 96-Well anti-Rabbit Coated Plate: (Part No. 2004070) Two 96-well immuno-plates pre-coated with anti-Rabbit polyclonal antibody, sealed in a foil pouch.
- 2. Rabbit anti-cGMP polyclonal Antibody: (Part No. 2004761) One 25 µL vial.
- 3. cGMP Standard: (Part No. 2004762) One 2 mL bottle
- 4. Alkaline Phosphatase cGMP Tracer Conjugate: (Part No. 2004760) One 20 µL vial
- 5. 2x cGMP Assay Diluent: (Part No. 2004811) One 25 mL bottle.
- 6. 5x Wash Buffer: (Part No. 2004075) One 60 mL bottle.
- 7. Lysis Buffer: (Part No. 2004076) One 125 mL bottle.
- 8. 6x Alkaline Phosphatase Substrate: (Part No. 2004077) One 5 mL bottle.
Quality Assurance:
Routinely evaluated by ELISA.
Detection Methods:
Chemiluminescent
Storage Conditions:
1 year at 4°C from date of shipment