Product Images
Typical Met Standard Curve 100 µL of progressive 2 fold dilutions of the Met standard was run as described in the assay instructions.
Met STAR ELISA Kit
Description:
Met STAR ELISA Kit
Trade Name:
- STAR
- Upstate (Millipore)
Qty/Pk:
96 assays
Background Information:
Test Principle
The UPSTATE colorimetric STAR (Signal Transduction Assay Reaction) ELISA kit is a solid phase sandwich enzyme linked immunosorbent assay that provides a fast, sensitive method to detect specific levels of signaling targets in whole cell extracts. The Met plate is coated with a specific mouse monoclonal anti-Met capture antibody on the microwells of the 96-well clear plate. Sample lysate or the standard included in the kit are incubated in the microwells allowing Met antigen to be captured in the plate wells. The plate is then washed to remove any non-bound unspecific material. The wells are then incubated with a specific rabbit anti-Met antibody to detect the captured Met on the plate well. The unbound detection antibody is washed away followed by incubation with an HRP-conjugated anti-rabbit antibody. This allows for a sensitive enzymatic detection of the sample. After the addition of TMB substrate and stop solution the absorbance is measured at 450 nm using a plate reader. The entire assay takes less than 5 hours to complete with minimal hands-on time. Many of the reagents are supplied in ready-to use formulations for ease of use. The kit also includes a standard that is run as both a positive control and to develop a standard curve.
Met Background
Met is a tyrosine kinase receptor for Hepatocyte Growth Factor (HGF), thought to stimulate multiple cellular processes including cell proliferation, differentiation, cell migration and tumorigenesis. Chronic stimulation of Met on cancer cells is thought to play a role in metastasis.
The UPSTATE colorimetric STAR (Signal Transduction Assay Reaction) ELISA kit is a solid phase sandwich enzyme linked immunosorbent assay that provides a fast, sensitive method to detect specific levels of signaling targets in whole cell extracts. The Met plate is coated with a specific mouse monoclonal anti-Met capture antibody on the microwells of the 96-well clear plate. Sample lysate or the standard included in the kit are incubated in the microwells allowing Met antigen to be captured in the plate wells. The plate is then washed to remove any non-bound unspecific material. The wells are then incubated with a specific rabbit anti-Met antibody to detect the captured Met on the plate well. The unbound detection antibody is washed away followed by incubation with an HRP-conjugated anti-rabbit antibody. This allows for a sensitive enzymatic detection of the sample. After the addition of TMB substrate and stop solution the absorbance is measured at 450 nm using a plate reader. The entire assay takes less than 5 hours to complete with minimal hands-on time. Many of the reagents are supplied in ready-to use formulations for ease of use. The kit also includes a standard that is run as both a positive control and to develop a standard curve.
Met Background
Met is a tyrosine kinase receptor for Hepatocyte Growth Factor (HGF), thought to stimulate multiple cellular processes including cell proliferation, differentiation, cell migration and tumorigenesis. Chronic stimulation of Met on cancer cells is thought to play a role in metastasis.
Key Applications:
ELISA
Species Reactivity:
Human
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Entrez Gene Summary:
The proto-oncogene MET product is the hepatocyte growth factor receptor and encodes tyrosine-kinase activity. The primary single chain precursor protein is post-translationally cleaved to produce the alpha and beta subunits, which are disulfide linked to form the mature receptor. Various mutations in the MET gene are associated with papillary renal carcinoma.
UniProt Summary:
FUNCTION: SwissProt: P08581 # Receptor for hepatocyte growth factor and scatter factor. Has a tyrosine-protein kinase activity.
SIZE: 1390 amino acids; 155527 Da
SUBUNIT: Heterodimer formed of an alpha chain (50 kDa) and a beta chain (145 kDa) which are disulfide linked. Binds PLXNB1 and GRB2. Interacts with SPSB1, SPSB2 and SPSB4 (By similarity). Interacts with INPP5D/SHIP1. When phosphorylated at Tyr-1356, interacts with INPPL1/SHIP2. Interacts with RANBP9 and RANBP10.
SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
DISEASE: SwissProt: P08581 # Activation of MET after rearrangement with the TPR gene produces an oncogenic protein. & Defects in MET may be associated with gastric cancer. & Defects in MET are a cause of hepatocellular carcinoma (HCC) [MIM:114550]. & Defects in MET are a cause of hereditary papillary renal carcinoma (HPRC) [MIM:605074]; also known as papillary renal cell carcinoma 2 (RCCP2). HPRC is a form of inherited kidney cancer characterized by a predisposition to develop multiple, bilateral papillary renal tumors. The pattern of inheritance is consistent with autosomal dominant transmission with reduced penetrance. & Genetic variations in MET may be associated with susceptibility to autism type 1B (AUTS1B) [MIM:608636]. Autism is a neurodevelopmental disorder characterized by disturbance in language, perception and socialization. The disorder is classically defined by a triad of limited or absent verbal communication, a lack of reciprocal social interaction or responsiveness, and restricted, stereotypical, and ritualized patterns of interests and behavior.
SIMILARITY: SwissProt: P08581 ## Belongs to the protein kinase superfamily. Tyr protein kinase family. & Contains 3 IPT/TIG domains. & Contains 1 protein kinase domain. & Contains 1 Sema domain.
SIZE: 1390 amino acids; 155527 Da
SUBUNIT: Heterodimer formed of an alpha chain (50 kDa) and a beta chain (145 kDa) which are disulfide linked. Binds PLXNB1 and GRB2. Interacts with SPSB1, SPSB2 and SPSB4 (By similarity). Interacts with INPP5D/SHIP1. When phosphorylated at Tyr-1356, interacts with INPPL1/SHIP2. Interacts with RANBP9 and RANBP10.
SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
DISEASE: SwissProt: P08581 # Activation of MET after rearrangement with the TPR gene produces an oncogenic protein. & Defects in MET may be associated with gastric cancer. & Defects in MET are a cause of hepatocellular carcinoma (HCC) [MIM:114550]. & Defects in MET are a cause of hereditary papillary renal carcinoma (HPRC) [MIM:605074]; also known as papillary renal cell carcinoma 2 (RCCP2). HPRC is a form of inherited kidney cancer characterized by a predisposition to develop multiple, bilateral papillary renal tumors. The pattern of inheritance is consistent with autosomal dominant transmission with reduced penetrance. & Genetic variations in MET may be associated with susceptibility to autism type 1B (AUTS1B) [MIM:608636]. Autism is a neurodevelopmental disorder characterized by disturbance in language, perception and socialization. The disorder is classically defined by a triad of limited or absent verbal communication, a lack of reciprocal social interaction or responsiveness, and restricted, stereotypical, and ritualized patterns of interests and behavior.
SIMILARITY: SwissProt: P08581 ## Belongs to the protein kinase superfamily. Tyr protein kinase family. & Contains 3 IPT/TIG domains. & Contains 1 protein kinase domain. & Contains 1 Sema domain.
Quality Assurance:
Routinely evaluated by ELISA.
Components:
- 1. Capture Plate pre-coated with anti-human Met antibody: (Part No. 17-469A) One pre-coated 96-stripwell immunoplate sealed in a foil pouch.
- 2. Anti-Met detection antibody: (Part No. 17-469B) One bottle (11 mL) of anti-Met detection antibody containing sodium azide, ready to use.
- 3. ELISA Diluent: (Part No. 17-469C) One bottle (25 mL) of ELISA Diluent containing sodium azide, ready to use.
- 4. 25X ELISA Wash Buffer: (Part No. 17-469D) One bottle (50 mL) of 25X ELISA Wash Buffer.
- 5. Anti-Rabbit IgG HRP conjugate: (Part No. 17-469E) One vial (125 µL) of 100X anti-rabbit HRP conjugate containing thimerosol.
- 6. HRP Diluent: (Part No. 17-469F) One bottle (25 mL) of HRP Diluent containing thimerosol.
- 7. TMB Solution: (Part No. 17-469G) One bottle (25 mL) of stabilized tetramethylbenzidine (TMB), ready to use.
- 8. Stop Solution: (Part No. 17-469H) One bottle (25 mL) of stop solution, ready to use.
- 9. Met Standard: (Part No. 17-469I) Two vials of Met standard, lyophilized.
- 10. Plate Covers: Two plate covers.
Brand Family:
Upstate
Sensitivity:
Sensitivity: 0.4 ng/mL.
Range of Detection: 0.4 to 25 ng/mL
Range of Detection: 0.4 to 25 ng/mL
UniProt Number:
Storage Conditions:
1 year at 4°C
Detection Methods:
- Chromogenic
- Chromogenic
Gene Symbol:
- MET
- HGFR
- AUTS9
- c-Met
- RCCP2
Product Name:
Met STAR ELISA Kit
Entrez Gene Number:
Materials Required but Not Delivered:
1. Multi-channel or repeating pipettes
2. Plate shaker (optional)
3. Pipettors & tips capable of accurately measuring 1-1000 µL
4. Graduated serological pipettes
5. 96-well microtiter Plate Reader with 450 nm filter
6. Graphing software for plotting data or graph paper for manual plotting of data
7. Microfuge tubes for standard and sample dilutions
8. Mechanical vortex
9. 1 liter container
10. Distilled or deionized water
2. Plate shaker (optional)
3. Pipettors & tips capable of accurately measuring 1-1000 µL
4. Graduated serological pipettes
5. 96-well microtiter Plate Reader with 450 nm filter
6. Graphing software for plotting data or graph paper for manual plotting of data
7. Microfuge tubes for standard and sample dilutions
8. Mechanical vortex
9. 1 liter container
10. Distilled or deionized water
Analytes Available:
Met