STAR MEK1 ELISA Kit

STAR MEK1 ELISA Kit

• STAR
• Upstate (Millipore)

The UPSTATE colorimetric STAR (Signal Transduction Assay Reaction) ELISA kit is a solid phase sandwich enzyme linked immunosorbent assay that provides a fast, sensitive method to detect specific levels of signaling targets in denatured cell extracts. The MEK1 plate is coated with a specific mouse monoclonal anti-MEK1 capture antibody on the microwells of the 96-well clear plate. Sample lysate or the standard included in the kit are incubated in the microwells allowing MEK1 antigen to be captured in the plate wells. The plate is then washed to remove any non-bound unspecific material. The wells are then incubated with a specific rabbit anti-MEK1 antibody to detect the captured MEK1 on the plate well. The unbound detection antibody is washed away followed by incubation with an HRP-conjugated anti-rabbit antibody. This allows for a sensitive enzymatic detection of the sample. After the addition of TMB substrate and stop solution the absorbance is measured at 450 nm using a plate reader. The entire assay takes less than 5 hours to complete with minimal hands-on time. Many of the reagents are supplied in ready-to use formulations for ease of use. The kit also includes a standard that is run as both a positive control and to develop a standard curve.

ELISA

• Human
• Mouse
• Rat

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Routinely evaluated by ELISA.

• Cell Signaling Kits
• ELISA Assays
• Kinase & Phosphatase Assays

• 1. Capture Plate pre-coated with anti-MEK1 antibody: (Part No. 17-473A) One pre-coated 96-stripwell immunoplate sealed in a foil pouch.
• 2. Anti-MEK1 detection antibody: (Part No. 17-473B) One bottle (11 mL) of anti-MEK1 detection antibody containing sodium azide, ready to use.
• 3. ELISA Diluent: (Part No. 17-473C) One bottle (25 mL) of ELISA Diluent containing sodium azide, ready to use.
• 4. 25X ELISA Wash Buffer: (Part No. 17-473D) One bottle (50 mL) of 25X ELISA Wash Buffer.
• 5. Anti-Rabbit IgG HRP conjugate: (Part No. 17-473E) One vial (125 µL) of 100X anti-rabbit HRP conjugate containing thimerosol.
• 6. HRP Diluent: (Part No. 17-473F) One bottle (25 mL) of HRP Diluent containing thimerosol.
• 7. TMB Solution: (Part No. 17-473G) One bottle (25 mL) of stabilized tetramethylbenzidine (TMB), ready to use.
• 8. Stop Solution: (Part No. 17-473H) One bottle (25 mL) of stop solution, ready to use.
• 9. MEK1 Standard: (Part No. 17-473I) Two vials of MEK1 standard, lyophilized.
• 10. Sample Incubation Buffer: (Part No. 17-73J) One bottle (10 mL) of a sample incubation (denaturing) buffer, ready to use.
• 11. Plate Covers: Two plate covers.

Sensitivity: 0.16 ng/mL
Range of Detection: 0.16 to 10 ng/mL

1 year at 4°C

• Chromogenic
• Chromogenic

• MAP2K1
• MAPKK1
• PRKMK1
• MEK1
• MKK1

1. Multi-channel or repeating pipettes
2. Plate shaker (optional)
3. Pipettors & tips capable of accurately measuring 1-1000 µL
4. Graduated serological pipettes
5. 96-well microtiter Plate Reader with 450 nm filter
6. Graphing software for plotting data or graph paper for manual plotting of data
7. Microfuge tubes for standard and sample dilutions
8. Mechanical vortex
9. 1 liter container
10. Distilled or deionized water

MEK1