STAR phospho-MEK1 (Ser218/222) ELISA Kit

STAR phospho-MEK1 (Ser218/222) ELISA Kit

• STAR
• Upstate (Millipore)

96 assays

The UPSTATE colorimetric STAR (Signal Transduction Assay Reaction) ELISA kit is a solid phase sandwich enzyme linked immunosorbent assay that provides a fast, sensitive method to detect specific levels of signaling targets in denatured cell extracts. The MEK1 plate is coated with a specific mouse monoclonal anti-MEK1 capture antibody on the microwells of the 96-well clear plate. Sample lysate or the standard included in the kit are incubated in the microwells allowing MEK1 antigen to be captured in the plate wells. The plate is then washed to remove any non-bound unspecific material. The wells are then incubated with a specific rabbit anti-phospho-MEK1 (Ser218/222) antibody to detect the captured on the plate well. The unbound detection antibody is washed away followed by incubation with an HRP-conjugated anti-rabbit antibody. This allows for a sensitive enzymatic detection of the sample. After the addition of TMB substrate and stop solution the absorbance is measured at 450 nm using a plate reader. The entire assay takes less than 5 hours to complete with minimal hands-on time. Many of the reagents are supplied in ready-to use formulations for ease of use. The kit also includes a standard that is run as both a positive control and to develop a standard curve.

ELISA

• Human
• Mouse

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

The protein encoded by this gene is a member of the dual specificity protein kinase family, which acts as a mitogen-activated protein (MAP) kinase kinase. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals. This protein kinase lies upstream of MAP kinases and stimulates the enzymatic activity of MAP kinases upon wide variety of extra- and intracellular signals. As an essential component of MAP kinase signal transduction pathway, this kinase is involved in many cellular processes such as proliferation, differentiation, transcription regulation and development.

FUNCTION: SwissProt: Q02750 # Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. Activates ERK1 and ERK2 MAP kinases.
SIZE: 393 amino acids; 43439 Da
SUBUNIT: Interacts with MORG1 (By similarity). Interacts with Yersinia yopJ.
PTM: Phosphorylation on Ser/Thr by MAP kinase kinase kinases (RAF or MEKK1) regulates positively the kinase activity. & Acetylation by Yersinia yopJ prevents phosphorylation and activation, thus blocking the MAPK signaling pathway.
DISEASE: SwissProt: Q02750 # Defects in MAP2K1 are a cause of cardiofaciocutaneous syndrome (CFC syndrome) [MIM:115150]; also known as cardio-facio- cutaneous syndrome. CFC syndrome is characterized by a distinctive facial appearance, heart defects and mental retardation. Heart defects include pulmonic stenosis, atrial septal defects and hypertrophic cardiomyopathy. Some affected individuals present with ectodermal abnormalities such as sparse, friable hair, hyperkeratotic skin lesions and a generalized ichthyosis-like condition. Typical facial features are similar to Noonan syndrome. They include high forehead with bitemporal constriction, hypoplastic supraorbital ridges, downslanting palpebral fissures, a depressed nasal bridge, and posteriorly angulated ears with prominent helices. The inheritance of CFC syndrome is autosomal dominant.
SIMILARITY: SwissProt: Q02750 ## Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily. & Contains 1 protein kinase domain.

Routinely evaluated by ELISA.

• 1. Capture Plate pre-coated with anti- MEK1 antibody: (Part No. 17-474A) One pre-coated 96-stripwell immunoplate sealed in a foil pouch.
• 2. Anti- phospho-MEK1 (Ser218/222) detection antibody: (Part No. 17-474B) One bottle (11 mL) of anti-phospho-MEK1 (Ser218/222) detection antibody containing sodium azide, ready to use.
• 3. ELISA Diluent: (Part No. 17-474C) One bottle (25 mL) of ELISA Diluent containing sodium azide, ready to use.
• 4. 25X ELISA Wash Buffer: (Part No. 17-474D) One bottle (50 mL) of 25X ELISA Wash Buffer.
• 5. Anti-Rabbit IgG HRP conjugate: (Part No. 17-474E) One vial (125 µL) of 100X anti-rabbit HRP conjugate containing thimerosol.
• 6. HRP Diluent: (Part No. 17-474F) One bottle (25 mL) of HRP Diluent containing thimerosol.
• 7. TMB Solution: (Part No. 17-474G) One bottle (25 mL) of stabilized tetramethylbenzidine (TMB), ready to use.
• 8. Stop Solution: (Part No. 17-474H) One bottle (25 mL) of stop solution, ready to use.
• 9. Phospho-MEK1 (Ser218/222 Standard: (Part No. 17-474I) Two vials of phospho-MEK1 (Ser218/222) standard, lyophilized.
• 10. Sample Incubation Buffer: (Part No. 17-73J) One bottle (10 mL) of a sample incubation (denaturing) buffer, ready to use.
• 11. Plate Covers: Two plate covers.

Upstate

Sensitivity: 0.75 Units/mL.
Range of Detection: 1.6 to 100 Units/mL

Q02750

2 years at 4°C

• Chromogenic
• Chromogenic

Phosphorylation

• MAP2K1
• MAPKK1
• PRKMK1
• MEK1
• MKK1

STAR phospho-MEK1 (Ser218/222) ELISA Kit

NM_002755.2

1. Multi-channel or repeating pipettes
2. Plate shaker (optional)
3. Pipettors & tips capable of accurately measuring 1-1000 %micro;L
4. Graduated serological pipettes
5. 96-well microtiter Plate Reader with 450 nm filter
6. Graphing software for plotting data or graph paper for manual plotting of data
7. Microfuge tubes for standard and sample dilutions
8. Mechanical vortex
9. 1 liter container
10. Distilled or deionized water

MEK1