Catch and Release® v2.0 Reversible Immunoprecipitation System
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Immunoprecipitation
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IP-Kinase Assay
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Western Blotting
Catch and Release® v2.0 should not be used with proteins containing a 6-His tag.
All
All components to be stored at 4°C except the Catch and Release® Capture Tubes which are stored at room temperature. Components are stable for 6 months from date of shipment.
routinely evaluated by immunoprecipitation.
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Catch and Release® Wash Buffer, 10X (Cat.# 20-210)
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Catch and Release® Non-denaturing Elution Buffer, 4X (Cat.# 20-209)
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Catch and Release® Denaturing Elution Buffer, 1X (Cat.# 20-284)
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20-285
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20-216
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Catch and Release® Capture Tubes
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Catch and Release
Kit capacity: 50 immunoprecipitations
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Western Blotting Kits
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Immunoprecipitation kits
Features & Benefits:
Reproducible: eliminates aspiration steps that typically lead to diminished/lost sample
Convenient: spin-column format dramatically simplifies sample handling
Reversible: simple elution of antigen:antibody complex in either native or denatured form
Use:
This kit allows for quick and reproducible immunoprecipitation (IP) by using a spin column. The system is more reproducible than regular IP's, which are problematic with regards to washing the protein A/G agarose without disrupting the agarose bed. The binding of the antibody/antigen complex in Catch and Release® is reversible, and elution of the immune complex can occur with native or denaturing buffers. The system has been tested successfully with rabbit, mouse, sheep and goat antibodies. IP using human IgG1-4 should be suitable. IP using chicken antibodies or human IgA, IgD, IgE or IgM is not recommended with this kit. Please read the enclosed product manual before use.
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Immunoprecipitation Reagents
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Western Blotting Reagents
Immunoprecipitation Assays
