Product Images
Catch and Release® columns and protocol were used with the Denaturing Elution Buffer to immunoprecipitate cdk2. HeLa nuclear extract was mixed with An...
 or normal rabbit IgG (Panel B) as a negative control for 1 hour at room temperature. Samples from each fraction were run on an SDS-PAGE gel and immunoblotted. The upper band is the heavy chain of IgG and the lower band is cdk2. Lane 1: flow through; Lane 2: wash 1; Lane 3: wash 2; Lane 4: wash 3; Lane 5: elution 1; Lane 6: elution 2; Lane 7: elution 3; Lane 8: Anti-cdk2; Lane 9: HeLa nuclear extract.')){kind=spacer}
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Catch and Release® v2.0 Reversible Immunoprecipitation System
Description:
Catch and Release® v2.0 Reversible Immunoprecipitation System
Trade Name:
- Catch & Release
- Upstate (Millipore)
Qty/Pk:
1 kit
Product Overview:
Features & Benefits:
Reproducible: eliminates aspiration steps that typically lead to diminished/lost sample
Convenient: spin-column format dramatically simplifies sample handling
Reversible: simple elution of antigen:antibody complex in either native or denatured form
Use:
This kit allows for quick and reproducible immunoprecipitation (IP) by using a spin column. The system is more reproducible than regular IP's, which are problematic with regards to washing the protein A/G agarose without disrupting the agarose bed. The binding of the antibody/antigen complex in Catch and Release® is reversible, and elution of the immune complex can occur with native or denaturing buffers. The system has been tested successfully with rabbit, mouse, sheep and goat antibodies. IP using human IgG1-4 should be suitable. IP using chicken antibodies or human IgA, IgD, IgE or IgM is not recommended with this kit. Please read the enclosed product manual before use.
Reproducible: eliminates aspiration steps that typically lead to diminished/lost sample
Convenient: spin-column format dramatically simplifies sample handling
Reversible: simple elution of antigen:antibody complex in either native or denatured form
Use:
This kit allows for quick and reproducible immunoprecipitation (IP) by using a spin column. The system is more reproducible than regular IP's, which are problematic with regards to washing the protein A/G agarose without disrupting the agarose bed. The binding of the antibody/antigen complex in Catch and Release® is reversible, and elution of the immune complex can occur with native or denaturing buffers. The system has been tested successfully with rabbit, mouse, sheep and goat antibodies. IP using human IgG1-4 should be suitable. IP using chicken antibodies or human IgA, IgD, IgE or IgM is not recommended with this kit. Please read the enclosed product manual before use.
Key Applications:
- Immunoprecipitation
- IP-Kinase Assay
- Western Blotting
Application Notes:
Catch and Release® v2.0 should not be used with proteins containing a 6-His tag.
Species Reactivity:
All
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Quality Assurance:
routinely evaluated by immunoprecipitation.
Components:
- Catch and Release® Wash Buffer, 10X (Cat.# 20-210)
- Catch and Release® Non-denaturing Elution Buffer, 4X (Cat.# 20-209)
- Catch and Release® Denaturing Elution Buffer, 1X (Cat.# 20-284)
- 20-285
- 20-216
- Catch and Release® Capture Tubes
Brand Family:
Upstate
Storage Conditions:
All components to be stored at 4°C except the Catch and Release® Capture Tubes which are stored at room temperature. Components are stable for 6 months from date of shipment.
Packaging:
50 immunoprecipitations
Product Name:
Catch and Release® v2.0 Reversible Immunoprecipitation System