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Catch and Release® columns and protocol were used with the Denaturing Elution Buffer to immunoprecipitate cdk2. HeLa nuclear extract was mixed with An...
Catch and Release® v2.0 Reversible Immunoprecipitation System
Description:
Catch and Release® v2.0 Reversible Immunoprecipitation System
Trade Name:
- Catch & Release
- Upstate (Millipore)
Product Overview:
Features & Benefits:
Reproducible: eliminates aspiration steps that typically lead to diminished/lost sample
Convenient: spin-column format dramatically simplifies sample handling
Reversible: simple elution of antigen:antibody complex in either native or denatured form
Reproducible: eliminates aspiration steps that typically lead to diminished/lost sample
Convenient: spin-column format dramatically simplifies sample handling
Reversible: simple elution of antigen:antibody complex in either native or denatured form
Species Reactivity:
All
Application Notes:
Catch and Release® v2.0 should not be used with proteins containing a 6-His tag.
Quality Assurance:
routinely evaluated by immunopreciptation
Storage Conditions:
6 months at 4°C from date of shipment
Components:
- Catch and Release® v2.0 Spin Columns
- Antibody Capture Affinity Ligand
- Catch and Release® Wash Buffer, 10X
- Non-denaturing Elution Buffer, 4X
- Denaturing Elution Buffer, 1X
- Catch and Release® Capture Tubes
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Key Applications:
- Immunoprecipitation
- IP-Kinase Assay
Qty/Pk:
1 kit
Brand Family:
Upstate
Packaging:
5 immunoprecipitations
Reagent Type:
- Immunoprecipitation Reagents
- Western Blotting Reagents
Product Name:
Catch and Release® v2.0 Reversible Immunoprecipitation System
Chromatography Product Type:
Magnetic Beads