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CHO wt cells were seeded at 30,000 cells per well in a 96-well plate 24 hr before the assay. Cells were pre-incubated with 1 mM IBMX for 5 minutes, t...
ChemiScreen™ β2 cells were spun down and resuspended in HBSS buffer with Ca++ and Mg++ containing 1mM IBMX at 2x10exp6 cells/mL. 100,000 cells/well (5...
cAMP HTS Immunoassay Kit (Chemiluminescent)
Description:
cAMP HTS Immunoassay Kit (Chemiluminescent)
Trade Name:
Upstate (Millipore)
Background Information:
Upstate®'s cAMP HTS Immunoassay kit is a competitive immunoassay for highly sensitive and rapid chemiluminescent quantitation of cyclic AMP from cell extracts of any species. The kit comprises a specific anti-cAMP antibody that recognizes all species, an alkaline phosphatase (AP)-labeled cAMP conjugate, ten 96 well microplates pre-coated with an anti-Rabbit antibody, cAMP standard, and an Alkaline Phosphatase chemiluminescence substrate
Key Applications:
ELISA
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Quality Assurance:
Routinely evaluated by ELISA.
Components:
- 10 White 96-well anti-Rabbit Coated Plate
- Rabbit anti-cAMP antibody
- cAMP standard
- cAMP Alkaline Phosphatase Conjugate Tracer
- 2X Assay Diluent
- 5X Wash Buffer
- Lysis Buffer
- 6x Alkaline Phosphatase Substrate
Storage Conditions:
Maintain the unopened kit at 2° to 8°C until the expiration date indicated on the label. After opening the kit, maintain the White 96-Well anti-Rabbit Coated Plates, cAMP Alkaline Phosphatase Conjugate Tracer, cAMP Standard, 5x Wash Buffer, 2x Assay Diluent, Lysis Buffer, and Alkaline Phosphatase Substrate at 2° to 8°C until the expiration date indicated on the label. For long-term storage, maintain the Rabbit anti-cAMP Polyclonal Antibody at -20°C.
Packaging:
Kit capacity: 960 wells
Materials Required but Not Delivered:
• Multi-channel or repeating pipettes
• Pipettors & tips capable of accurately measuring 10-1000 μL
• Plate shaker (optional)
• Automated Liquid Handler (optional)
• Plate Washer (optional)
• Luminometer capable of reading 96-well microplates.
• Test tubes for standard and sample dilutions
• Mechanical vortex
• Pipettors & tips capable of accurately measuring 10-1000 μL
• Plate shaker (optional)
• Automated Liquid Handler (optional)
• Plate Washer (optional)
• Luminometer capable of reading 96-well microplates.
• Test tubes for standard and sample dilutions
• Mechanical vortex