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HeLa nuclear extract was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-Sp1 (0.2 μg/ml). Proteins were visualized usi...
ChIP Analysis:
Representative lot data.
Sonicated chromatin prepared from 1 x 106 Hela cells was subjected to chromatin ...
ChIPAb+ Sp1
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M, R | Chromatin Immunoprecipitation (ChIP), WB | Rabbit | Purified | Polyclonal Antibody |
Description:
ChIPAb+ Sp1
Trade Name:
Upstate (Millipore)
Product Overview:
Every lot of the ChIPAb+ line of antibodies is individually validated for chromatin precipitation, in order to guarantee successful ChIP assays every time. Each antibody includes a control primer set for performance confirmation. Sp1 antibody and the negative control antibody (rabbit normal IgG) can be used to demonstrate that the Sp1 antibody is functionally validated in the precipitation of Sp1 associated chromatin.
The qPCR primers included flank the Sp1 binding site in human DHFR promoter.
The qPCR primers included flank the Sp1 binding site in human DHFR promoter.
Specificity:
Sp1
Molecular Weight:
105 kDa and 95 kDa
Immunogen:
Full length human Sp1 protein
Species Reactivity:
- Human
- Mouse
- Rat
Application Notes:
Western Blot Analysis:
HeLa nuclear extract was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-Sp1 (0.2 μg/mL). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system (Please see figures).
HeLa nuclear extract was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-Sp1 (0.2 μg/mL). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system (Please see figures).
Control:
Includes negative control rabbit IgG and control primers specific for human dihydrofolate reductase (DHFR) promoter.
Quality Assurance:
Chromatin immunoprecipitation:
Sonicated chromatin prepared from 1 x 106 HeLa cells was subjected to chromatin immunoprecipitation using 4 μg of either the negative control antibody, Rabbit Normal IgG or Rabbit Anti-Sp1 and the Magna ChIPTM A kit (Cat.# 17-610) Rapid Protocol (2 hour IP). Successful enrichment of Sp1 associated DNA fragments was verified by qPCR using ChIP Primers DHFR flanking the human DHFR promoter that contains a Sp1 binding site (Please see figures).
Please refer to the EZ-Magna ChIP™ A (Cat.# 17-408)
or EZ-ChIP™ (Cat.# 17-371) kit protocols for
experimental details.
Sonicated chromatin prepared from 1 x 106 HeLa cells was subjected to chromatin immunoprecipitation using 4 μg of either the negative control antibody, Rabbit Normal IgG or Rabbit Anti-Sp1 and the Magna ChIPTM A kit (Cat.# 17-610) Rapid Protocol (2 hour IP). Successful enrichment of Sp1 associated DNA fragments was verified by qPCR using ChIP Primers DHFR flanking the human DHFR promoter that contains a Sp1 binding site (Please see figures).
Please refer to the EZ-Magna ChIP™ A (Cat.# 17-408)
or EZ-ChIP™ (Cat.# 17-371) kit protocols for
experimental details.
Purification Method:
Protein A purfied
Presentation:
Anti-Sp1 (Rabbit polyclonal IgG). 1 vial containing 100 μg of protein A purified IgG in 100 μL of 0.014 M phosphate buffer, pH 7.6, 0.175 M NaCl, 0.07% sodium azide and 30% glycerol. Store at -20°C. The Sp1 antibody is made against full length human Sp1 protein and can recognize Sp1, MW 105 kDa and 95 kDa, of human, mouse and rat origins.
Normal Rabbit IgG. One vial containing 125 μg of normal rabbit IgG in 125 μL. Store at -20°C.
ChIP primers DHFR. 1 vial containing 75 μL of 5 μM of each control primer specific for human dihydrofolate reductase (DHFR) promoter. Store at -20°C.
FOR: TCG CCT GCA CAA ATA GGG AC
REV: AGA ACG CGC GGT CAA GTT T
Normal Rabbit IgG. One vial containing 125 μg of normal rabbit IgG in 125 μL. Store at -20°C.
ChIP primers DHFR. 1 vial containing 75 μL of 5 μM of each control primer specific for human dihydrofolate reductase (DHFR) promoter. Store at -20°C.
FOR: TCG CCT GCA CAA ATA GGG AC
REV: AGA ACG CGC GGT CAA GTT T
Storage Conditions:
1 year at -20°C from date of shipment.
UniProt Number:
Entrez Gene Number:
Gene Symbol:
- SP1
- TSFP1
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Key Applications:
- Chromatin Immunoprecipitation (ChIP)
- Western Blotting
UniProt Summary:
FUNCTION: SwissProt: P08047 # Binds to GC box promoters elements and selectively activates mRNA synthesis from genes that contain functional recognition sites. Can interact with G/C-rich motifs from serotonin receptor promoter.
SIZE: 785 amino acids; 80693 Da
SUBUNIT: Interacts with ATF7IP, ATF7IP2, POGZ, HCFC1, AATF and PHC2. Interacts with varicella-zoster virus IE62 protein and HIV-1 Vpr.
SUBCELLULAR LOCATION: Nucleus.
PTM: O-glycosylated; contains N-acetylglucosamine side chains.
SIMILARITY: SwissProt: P08047 ## Belongs to the Sp1 C2H2-type zinc-finger protein family. & Contains 3 C2H2-type zinc fingers.
SIZE: 785 amino acids; 80693 Da
SUBUNIT: Interacts with ATF7IP, ATF7IP2, POGZ, HCFC1, AATF and PHC2. Interacts with varicella-zoster virus IE62 protein and HIV-1 Vpr.
SUBCELLULAR LOCATION: Nucleus.
PTM: O-glycosylated; contains N-acetylglucosamine side chains.
SIMILARITY: SwissProt: P08047 ## Belongs to the Sp1 C2H2-type zinc-finger protein family. & Contains 3 C2H2-type zinc fingers.
Qty/Pk:
25 assays
Brand Family:
Upstate
Format:
Purified
Host:
Rabbit
Packaging:
25 assays per kit, ~4μg per chromatin immunoprecipitation.
Product Name:
ChIPAb+ Sp1
Antibody Type:
Polyclonal Antibody