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HeLa nuclear extract was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-Sp1 (0.2 μg/ml). Proteins were visualized usi...
ChIPAb+ Sp1
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M, R | Chromatin Immunoprecipitation (ChIP), WB | Rabbit | null | Polyclonal Antibody |
UniProt Number:
UniProt Summary
FUNCTION: SwissProt: P08047 # Binds to GC box promoters elements and selectively activates mRNA synthesis from genes that contain functional recognition sites. Can interact with G/C-rich motifs from serotonin receptor promoter.
SIZE: 785 amino acids; 80693 Da
SUBUNIT: Interacts with ATF7IP, ATF7IP2, POGZ, HCFC1, AATF and PHC2. Interacts with varicella-zoster virus IE62 protein and HIV-1 Vpr.
SUBCELLULAR LOCATION: Nucleus.
PTM: O-glycosylated; c ... see more »
SIZE: 785 amino acids; 80693 Da
SUBUNIT: Interacts with ATF7IP, ATF7IP2, POGZ, HCFC1, AATF and PHC2. Interacts with varicella-zoster virus IE62 protein and HIV-1 Vpr.
SUBCELLULAR LOCATION: Nucleus.
PTM: O-glycosylated; c ... see more »
Entrez Gene Number:
Description:
ChIPAb+ Sp1
Specificity:
Sp1
Key Applications:
- Chromatin Immunoprecipitation (ChIP)
- Western Blotting
Species Reactivity:
- Human
- Mouse
- Rat
Isotype:
IgG
Immunogen:
Full length human Sp1 protein
Molecular Weight:
105kDa and 95kDa
Control:
One vial containing 125μg of normal rabbit IgG in 125μl. Store at -20°C.
1 vial containing 75μl of 5μM of each control primer specific for human dihydrofolate reductase (DHFR) promoter. Store at -20°C.
FOR: TCG CCT GCA CAA ATA GGG AC
REV: AGA ACG CGC GGT CAA GTT T
1 vial containing 75μl of 5μM of each control primer specific for human dihydrofolate reductase (DHFR) promoter. Store at -20°C.
FOR: TCG CCT GCA CAA ATA GGG AC
REV: AGA ACG CGC GGT CAA GTT T
Presentation:
0.014M phosphate buffer, pH 7.6, 0.175M NaCl, 0.07% sodium azide and 30% glycerol.
Storage Conditions:
1 year at -20°C from date of shipment.
Gene Symbol:
- SP1
- TSFP1
Quality Assurance:
Routinely evaluated by chromatin immunoprecipitation and immunoblot on HeLa nuclear lysate.
Components:
- Anti-Sp1 polyclonal antibody, 1 vial
- Rabbit polyclonal antibody (negative control); 1 vial
- DHFR promoter primer set, 1 vial
Antibody Category:
Epigenetics & Nuclear Function
Antibody Sub-Category:
Chromatin Biology
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Trade Name:
Upstate (Millipore)
Antibody Type:
Polyclonal Antibody
Host:
Rabbit
Product Overview:
Every lot of the ChIPAb+ line of antibodies is individually validated for chromatin precipitation, in order to guarantee successful ChIP assays every time. Each antibody includes a control primer set for performance confirmation. Sp1 antibody and the negative control antibody (rabbit normal IgG) can be used to demonstrate that the Sp1 antibody is functionally validated in the precipitation of Sp1 associated chromatin.
The qPCR primers included flank the Sp1 binding site in human DHFR promoter.
The qPCR primers included flank the Sp1 binding site in human DHFR promoter.
Packaging:
25 assays per kit, ~4μg per chromatin immunoprecipitation.
Purification Method:
protein A purified