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  • : 17-700
  • : 12 assays

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PCR Analysis of RIP (Fold Enrichment):
RIP was performed using HeLa cell lysate and either anti-SNRNP70 or Normal Rabbit IgG as the immunoprecipi...

Magna RIP™ RNA-Binding Protein Immunoprecipitation Kit

Description:
Magna RIP™ RNA-Binding Protein Immunoprecipitation Kit
Trade Name:
Upstate (Millipore)
Qty/Pk:
12 assays
Product Overview:
RNA-binding protein immunoprecipitation (RIP) is the RNA analog of the more well-known ChIP application (chromatin immunoprecipitation), which identifies DNA targets of DNA-binding proteins in an in-vivo cellular context. RIP can be used to identify specific RNA molecules (of many types) associated with specific nuclear or cytoplasmic binding proteins. These experiments involve immunoprecipitation of endogenously formed complexes of RNA-binding proteins and co-isolation of any RNA species associated with that RNA-binding protein. Purification of these RNA species allows interrogation and identification of mRNAs (and potentially non-coding RNAs associated with them) and can be directly measured using down stream applications including quantitative reverse transcription polymerase chain reaction (RT-PCR), microarray analysis (RIP-chip) and “deep-sequencing” or 2nd-generation sequencing based platforms (RIP-Seq).

Features & Benefits:
-Protein A/G magnetic beads, optimized to bind nucleic acid-protein immune complexes
-RNAse inhibitors and RNAse-free reagents
-Negative controls
Applications:
Magna RIP RNA-Binding Protein Immunoprecipitation Kit containing all necessary reagents to perform 12 individual RNA-binding protein immunoprecipitation (RIP) reactions.
Key Applications:
RNA Binding Protein Immunoprecipitation (RIP)
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Brand Family:
Upstate
Presentation:
Two boxes containing all necessary reagents to perform 12 individual RNA-binding protein immunoprecipitation (RIP) reactions.
Storage Conditions:
Upon receipt, store components at the temperatures indicated on the labels. Kit components are stable for 6 months from date of shipment when stored as directed.
Product Name:
Magna RIP™ RNA-Binding Protein Immunoprecipitation Kit
Materials Required but Not Delivered:
Magna Grip™ Rack 8 well (Cat.# 20-400) (Now Available!) or similar magnetic rack.
Components:
  • Magnetic Beads Protein A/G
  • RIP Wash Buffer
  • RIP Lysis Buffer
  • 0.5 M EDTA
  • 10% SDS
  • Salt Solution I
  • Salt Solution II
  • Precipitate Enhancer
  • Normal Mouse IgG
  • Rabbit IgG Purified
  • Protease Inhibitor Cocktail 200X
  • RNase Inhibitor
  • Proteinase K (10 mg/mL)
  • Nuclease free water
Packaging:
RIP Kit capacity: 12 RNA-binding protein immunoprecipitation assays