PKC Lipid Activator

PKC Lipid Activator

Upstate (Millipore)

1 mL

Kinase Assay

For use in kinase assays.

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Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. This kinase has been reported to play roles in many different cellular processes, such as cell adhesion, cell transformation, cell cycle checkpoint, and cell volume control. Knockout studies in mice suggest that this kinase may be a fundamental regulator of cardiac contractility and Ca(2+) handling in myocytes.

FUNCTION: SwissProt: P17252 # PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters.
COFACTOR: Binds 3 calcium ions per subunit. The ions are bound to the C2 domain (By similarity).
SIZE: 672 amino acids; 76764 Da
SUBUNIT: Interacts with CENTA1, CSPG4 and PRKCABP. Binds to SDPR in the presence of phosphatidylserine.
SIMILARITY: SwissProt: P17252 ## Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily. & Contains 1 AGC-kinase C-terminal domain. & Contains 1 C2 domain. & Contains 2 phorbol-ester/DAG-type zinc fingers. & Contains 1 protein kinase domain.

Upstate

20mM MOPS, pH 7.2, 25mM β-glycerolphosphate, 1mM sodium orthovanadate, 1mM dithiothreitol, 1mM CaCl2.

P17252

1 year at -20°C

• Buffers
• Agonists & Inhibitors

Members of the PKC family fall into three categories: conventional (PKC α, βI, βII, γ), Novel (δ [delta], ε [epsilon], η [eta], μ [mu]), and atypical (λ [lamda], ζ [zeta]). Conventional PKC isoforms are dependent on Ca²⁺ and diacylglycerol (DAG) for their activity, whereas novel PKC isoforms are Ca²⁺-independent, and atypical PKC isoforms are Ca²⁺- and DAG-independent. All PKC isoforms are dependent on membrane phospholipid, although some may become activated in a soluble form. PKC family members are broad-specificity kinases, which phosphorylate many proteins, the best known of which are MARCKS and nuclear lamins. The role of phorbol ester activators of PKC as tumor promoters clearly implicates PKC as activators of signaling pathways, including the MAP kinase pathway.

• PRKCA
• PKC-A
• PKCA
• PRKACA
• PKC-alpha
• MGC129901
• AAG6
• MGC129900

Tools for Enzyme Studies

PKC Lipid Activator

NM_002737.2