H-Ras cDNA (activated) in pUSEamp

H-Ras cDNA (activated) in pUSEamp

Upstate (Millipore)

5 µg

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FUNCTION: SwissProt: P01112 # Ras proteins bind GDP/GTP and possess intrinsic GTPase activity.
SIZE: 189 amino acids; 21298 Da
SUBUNIT: In its GTP-bound form interacts with PLCE1. Interacts with TBC1D10C. Interacts with RGL3 (By similarity).
SUBCELLULAR LOCATION: Cell membrane; Lipid-anchor; Cytoplasmic side. Golgi apparatus membrane; Lipid-anchor. Note=Shuttles between the plasma membrane and the Golgi apparatus.
PTM: Palmitoylated by the ZDHHC9-GOLGA7 complex. A continuous cycle of de- and re-palmitoylation regulates rapid exchange between plasma membrane and Golgi.
DISEASE: SwissProt: P01112 # Defects in HRAS are the cause of Costello syndrome [MIM:218040]; also known as faciocutaneoskeletal syndrome. Costello syndrome is a rare condition characterized by prenatally increased growth, postnatal growth deficiency, mental retardation, distinctive facial appearance, cardiovascular abnormalities (typically pulmonic stenosis, hypertrophic cardiomyopathy and/or atrial tachycardia), tumor predisposition, skin and musculoskeletal abnormalities. & Mutations which change positions 12, 13 or 61 activate the potential of c-ras to transform cultured cells and are implicated in a variety of human tumors. & Defects in HRAS are a cause of bladder cancer [MIM:109800]. & Defects in HRAS are also the cause of oral squamous cell carcinoma (OSCC).
SIMILARITY: SwissProt: P01112 ## Belongs to the small GTPase superfamily. Ras family.

Upstate

P01112

Ras proteins are small GTP-binding proteins which, unlike the heterotrimeric G-proteins, contain all GTPase and effector functions within a single polypeptide. At least three isoforms of Ras exist, Ki-Ras, Ha-Ras and N-Ras, with distinct expression patterns but similar signaling activity. Ras is palmitoylated and farnesylated at the carboxy terminus, anchoring it in the membrane. In resting cells, Ras is loaded with GDP, and is activated subsequent to growth factor stimulation of receptors, which recruit Ras Guanine nucleotide Exchange Factors to the plane of the membrane. Proximity of exchange factors to the Ras proteins causes release of GDP, and its replacement by GTP. In its GTP-bound form, Ras binds several proteins, including Raf, RalGDS and PI3 Kinase. Inactivation of Ras occurs by GTP hydrolysis, which is greatly accelerated by RasGAP or NF-1, two known Ras GTPase Activating Proteins. It is possible to assay for Ras activation by incubation of lysates with the Ras-binding domain of Raf-1, which selectively binds to Ras:GTP.

• HRAS
• c-bas/has
• H-Ras-1
• N-ras
• p21ras
• c-H-ras
• RASH1
• H-RasIDX
• Ha-Ras
• K-ras
• CTLO
• HRAS1

• NM_005343.2
• NM_176795.2

H-Ras cDNA (activated) in pUSEamp

cDNA Expression Plasmids