Ordering Information
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- : 1 kit
DetergentOUT™ GBS10
Description:
DetergentOUT™ GBS10
Trade Name:
Chemicon (Millipore)
Qty/Pk:
1 kit
Product Overview:
The Chemicon DetergentOUT™ GBS10 Kit is a simple high performance method for removing detergents from protein solutions. Removes detergents without significant dilution of protein solution. Simply load the protein solution on the DetergentOUT™ GBS10 Spin column and collect detergent free protein solution. Micro columns are suitable for removing detergent from a maximum of 0.2 mL sample volume.
Key Applications:
Protein Clean-up
Application Notes:
1. Invert the column several times to re-suspend the resin material. Remove the tip of the column and let the liquid drain off.
2. Buffer Equilibration: SpinOUT columns are supplied in deionized water. Equilibrate the column with a buffer of your choice, as follows. Note: Do not equilibrate the columns with borate buffers. Apply about 0.5 mL of a desired buffer into the column. Let the buffer drain into collection tube. Repeat this process 3 times.
3. Place the column in a 2.0 mL micro-centrifuge tube. Centrifuge at 1000 xg for 20-30 seconds. Discard the liquid collected in the centrifuge tube.
4. Place the column back in the same centrifuge tube. Carefully apply the protein suspension to the center of the column. Wait for 5 minutes. Note: If your protein solution is in borate buffer, exchange the protein solution buffer to phosphate or other buffer, using either a SpinOUT columns or by dialysis.
5. After loading the column centrifuge at 1000 xg for 20-30 seconds. Collect the liquid comprising purified protein material free from GBS that originally contained in the sample.
Detecting Efficiency of GBS Removal:
Mix 1-5 μL of sample with 2 mL Blue Dye and 1 mL Dye Extraction Buffer. Add 2 mL of chloroform into the mix. Mix the tube content by inverting the tube 5-6 times. Allow the tube to stand 5 minutes. If the chloroform layer extract color is blue, it is indicative of the presence of GBS in the sample. Measure the optical density of the chloroform layer at 600 nm.
By comparing the optical density of the chloroform layers before and after you can determine the efficiency of GBS removal.
2. Buffer Equilibration: SpinOUT columns are supplied in deionized water. Equilibrate the column with a buffer of your choice, as follows. Note: Do not equilibrate the columns with borate buffers. Apply about 0.5 mL of a desired buffer into the column. Let the buffer drain into collection tube. Repeat this process 3 times.
3. Place the column in a 2.0 mL micro-centrifuge tube. Centrifuge at 1000 xg for 20-30 seconds. Discard the liquid collected in the centrifuge tube.
4. Place the column back in the same centrifuge tube. Carefully apply the protein suspension to the center of the column. Wait for 5 minutes. Note: If your protein solution is in borate buffer, exchange the protein solution buffer to phosphate or other buffer, using either a SpinOUT columns or by dialysis.
5. After loading the column centrifuge at 1000 xg for 20-30 seconds. Collect the liquid comprising purified protein material free from GBS that originally contained in the sample.
Detecting Efficiency of GBS Removal:
Mix 1-5 μL of sample with 2 mL Blue Dye and 1 mL Dye Extraction Buffer. Add 2 mL of chloroform into the mix. Mix the tube content by inverting the tube 5-6 times. Allow the tube to stand 5 minutes. If the chloroform layer extract color is blue, it is indicative of the presence of GBS in the sample. Measure the optical density of the chloroform layer at 600 nm.
By comparing the optical density of the chloroform layers before and after you can determine the efficiency of GBS removal.
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Components:
DetergentOUT™ GBS10 Column 10 Micro
Brand Family:
Chemicon
Storage Conditions:
Store at 2-8°C for up to 6 months from date of purchase. To avoid possible microbial contamination, dispense all solutions aseptically.
Packaging:
10 columns
Reagent Type:
Detergents
Product Name:
DetergentOUT™ GBS10
Materials Required but Not Delivered:
Chloroform
Centrifuge
Centrifuge tubes
Collection tubes
Centrifuge
Centrifuge tubes
Collection tubes