Ordering Information
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- : 1 kit
Detergent-OUT™ Med Kit
Description:
Detergent-OUT™ Med Kit
Trade Name:
Chemicon (Millipore)
Qty/Pk:
1 kit
Product Overview:
The Chemicon Detergent-OUT Kit is a simple high performance method for removing detergents from protein solutions. Removes detergents without significant dilution of protein solution. Simply load the protein solution on the Detergent-OUT Spin column and collect detergent free protein solution. The Med column is suitable for removing detergents from a 1.0 mL solution of 1.5% Triton-X100 detergents (or an equivalent of 15 μL Triton-X100 or 15 mg detergents), provided that both the columns and proteins solutions are prepared in water.
Key Applications:
Protein Clean-up
Application Notes:
IMPROVING DETERGENT REMOVAL EFFICIENCY: For samples in solutions other than water or phosphate buffer, better results are achieved if the sample is desalted first by gel filtration using a spin column, such as a Spin-OUT 6000 (see related product).
PROTOCOL:
1. Invert the column several times to re-suspend the resin material. Remove the tip of the column and let the liquid drain off.
2. Buffer Equilibration: Detergent-OUT columns are supplied in deionized water. A better result is obtained when the column and protein solution are in water or phosphate buffer. Columns may be equilibrated with other types of buffer. Note: Do not equilibrate the columns with Tris buffer and other such buffers. Apply about 1.0 mL of a desired buffer into the column. Let the buffer drain into collection tube. Repeat this process 3 times.
3. Place the column in a 15mL centrifuge tube. Centrifuge at 1000 xg for 20-30 seconds. Discard the liquid collected in the centrifuge tube.
4. Place the column back in the same centrifuge tube. Carefully apply the protein suspension to the center of the column. Wait for 5 minutes..
5. After loading the column centrifuge at 1000 xg for 20-30 seconds. Collect the liquid comprising purified protein material free from detergent originally present in the sample.
PROTOCOL:
1. Invert the column several times to re-suspend the resin material. Remove the tip of the column and let the liquid drain off.
2. Buffer Equilibration: Detergent-OUT columns are supplied in deionized water. A better result is obtained when the column and protein solution are in water or phosphate buffer. Columns may be equilibrated with other types of buffer. Note: Do not equilibrate the columns with Tris buffer and other such buffers. Apply about 1.0 mL of a desired buffer into the column. Let the buffer drain into collection tube. Repeat this process 3 times.
3. Place the column in a 15mL centrifuge tube. Centrifuge at 1000 xg for 20-30 seconds. Discard the liquid collected in the centrifuge tube.
4. Place the column back in the same centrifuge tube. Carefully apply the protein suspension to the center of the column. Wait for 5 minutes..
5. After loading the column centrifuge at 1000 xg for 20-30 seconds. Collect the liquid comprising purified protein material free from detergent originally present in the sample.
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Components:
Detergent-OUT Columns-DTG-100X 10 Med
Brand Family:
Chemicon
Storage Conditions:
Store at 2-8°C for up to 6 months from date of purchase. To avoid possible microbial contamination, dispense all solutions aseptically.
Packaging:
10 columns
Reagent Type:
Detergents
Product Name:
Detergent-OUT™ Med Kit
Materials Required but Not Delivered:
Centrifuge
Centrifuge tubes
Collection tubes
Centrifuge tubes
Collection tubes