Ordering Information
- : 23-021
- : 10 µg
Product Images
Activity was measured by titrating 112ng of UBE1 enzyme in a ubiquitination assay. Data shown for a representative lot.
Purity was assessed by SDS-PAGE and Coomassie blue staining using 3µg of active UBE1. Confirmed identity as UBE1 by mass spectrometry.
UBE1 Protein, active, 10 μg
Description:
UBE1, active
Trade Name:
Upstate (Millipore)
Qty/Pk:
10 µg
Background Information:
UBE1 catalyzes the first step in ubiquitin conjugation to mark cellular proteins for degradation. It is responsible for the primary ubiquitin-protein isopeptide bond formation and is a critical component for the initiation of in vitro conjugation reactions. UBE1 activates ubiquitin by the ATP dependent adenylation of its C-terminal glycine carboxyl group. This ubiquitin residue is then linked to the sulphydryl side chain moiety of a cysteine residue within UBE1 by forming a high energy thiol ester bond, liberating free AMP. There are two active sites within the UBE1 molecule allowing it to accommodate two ubiquitin molecules at one time, with a new ubiquitin forming an adenylate intermediate as the previous one is transferred to the thiol site. The activated ubiquitin is then transferred to the lysine of target proteins via the E2/E3 conjugation cascade.
Applications:
10 µg of UBE1. Active, full length, recombinant human UBE1. N-terminal 6His-tagged. For use in Enzyme Assays. Functions as an E1 enzyme in ubiquitination assays.
Key Applications:
Enzyme Assay
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Species:
Human
Cascade Component:
E1
Brand Family:
Upstate
Specific Activity:
For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
Long Description:
N-terminal 6His-tagged, recombinant human UBE1 full length, expressed by baculovirus in Sf21 insect cells.
Product Name:
UBE1, active
Entrez Gene Number:
Alternate Names:
- Ubiquitin activating enzyme E1
- Ubiquitin-like modifier-activating enzyme 1
- UBA1
- A1S9 Protein
- UBE1
Purification Method:
Purified using immobilized metal affinity chromatography.