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Ordering Information

  • : 23-022M
  • : 250 µg
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  • Product Family Information

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Activity was measured by titrating 116ng of UbcH3 enzyme in a ubiquitination assay. Data shown for a representative lot.

Purity was assessed by SDS-PAGE and Coomassie blue staining using 3µg of active UbcH3. Confirmed identity as UbcH3 by mass spectrometry.

UbcH3 Protein, active, 250 μg

Description:
UbcH3, active
Trade Name:
Upstate (Millipore)
Qty/Pk:
250 µg
Background Information:
Ubiquitin-conjugating (E2) enzymes are characterized by the presence of a highly conserved ubiquitin-conjugating domain which accommodates ATP-activated ubiquitin (Ub) via a covalently linked thioester onto its active-site residue. E2 enzymes act via selective protein-protein interactions with the ubiquitin-activating E1 enzyme and ubiquitin ligase E3 enzymes and are able to differentiate effects on downstream substrates, either with a single Ub molecule or an Ub chain. While E3s are involved in substrate selection, E2s are the main determinants for selection of the lysine to construct Ub chains, which thereby directly control the cellular fate of the substrate.
UbcH3 has been shown to interact with a number of different E3 ligases, including SCFSkp2/Cks1, promoting the ubiquitination of p27 and SCFβTrCP1, promoting the ubiquitination of β-catenin and IκBα.
Applications:
250 µg of UbcH3. Active, full length, recombinant human UbcH3. N-terminal HA, 6His-tagged. For use in Enzyme Assays. Functions as an E2 enzyme in ubiquitination assays.
Key Applications:
Enzyme Assay
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Species:
Human
Cascade Component:
E2
Brand Family:
Upstate
Specific Activity:
For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
Long Description:
N-terminal HA, 6His-tagged, recombinant human UbcH3 full length, expressed in E.coli.
Product Name:
UbcH3, active
Entrez Gene Number:
NM_004359 (GenBank Number)
Alternate Names:
  • E2-CDC34
  • UBE2R1
  • CDC34
  • Ubiquitin-protein ligase R1
  • Ubiquitin-conjugating enzyme E2 R1
Purification Method:
Purified using immobilized metal affinity chromatography.