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Ordering Information

  • : 23-023M
  • : 250 µg
  • Product Family Information

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Activity was measured by titrating 500ng of SCF Skp2/Cks1 complex in a ubiquitination assay. Data shown for a representative lot.

Purity was assessed by SDS-PAGE and Coomassie blue staining using 3µg of active SCFSkp2/Cks1 complex. Confirmed identity as Skp2, Skp1, Cul1, Rbx1 and...

SCF-Skp2 Protein Complex, Active, 250 μg

Description:
SCFSkp2 complex, active
Trade Name:
Upstate (Millipore)
Qty/Pk:
250 µg
Background Information:
The SCF (Skp1-Cul1-F-box protein) complexes represent the largest family of ubiquitin-protein ligases and mediate the ubiquitination of a broad spectrum of regulatory and signalling proteins in diverse cellular pathways. The SCF consists of three invariant components, Skp1, Cul1 and Rbx1 and an interchangeable subunit, an F-box protein which is responsible for recruiting specific substrates to be ubiquitinated by the SCF. SCFSkp2 mediates the ubiquitination and subsequent proteasomal degradation of target proteins involved in cell cycle progression, signal transduction and transcription. It specifically recognizes phosphorylated p27 and is involved in regulation of G1/S transition. Complex formation of SCFSkp2 with Cks1 is required for the degradation of p27. An increased level of Skp2 and reduced level of p27 is linked to poor prognosis in a number of cancers
Applications:
250 µg of SCFSkp2 complex. Active complex of five (5) full length, recombinant human enzymes: Skp1, Skp2, Cul1, Rbx1, and Cks1. For use in Enzyme Assays. Functions as an E3 ligase in ubiquitination assays.
Key Applications:
Enzyme Assay
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Species:
Human
Cascade Component:
E3
Brand Family:
Upstate
Specific Activity:
For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
Long Description:
Complex of N-terminal 6His-tagged, recombinant human Skp2 full length, N-terminal GST-tagged, recombinant human Skp1 full length, N-terminal 6His-tagged, recombinant human Cul1 full length and untagged recombinant human Rbx1 full length, co-expressed by baculovirus in Sf21 insect cells. Purified using glutathione sepharose and complexed in vitro with C-terminal 6His-tagged, recombinant human Cks1 full length expressed in E.coli
Product Name:
SCFSkp2 complex, active
Entrez Gene Number:
Alternate Names:
  • Skp2/Cks1 complex
  • SCFSkp2
Purification Method:
Purified using immobilized metal affinity chromatography.