Ordering Information
- : 23-024M
- : 250 µg
Product Images
Activity was measured by titrating 23ng of p27 protein complex in a ubiquitination assay. Data shown for a representative lot.
Purity was assessed by SDS-PAGE and Coomassie blue staining using 3µg of p27 complex, activated. Confirmed identity as p27, CDK2 and Cyclin E1 by mass...
p27 Protein complex, activated, 250 μg
Description:
p27 complex, activated
Trade Name:
Upstate (Millipore)
Qty/Pk:
250 µg
Background Information:
p27 is an important regulator of cell cycle progression, involved in G1 arrest. It is a potent inhibitor of cyclin E1- and cyclin A-CDK2 complexes and a positive regulator of cyclin D-dependent kinases such as CDK4. The degradation of this protein, which is triggered by its CDK-dependent phosphorylation and subsequent ubiquitination by SCF complexes, is required for the cellular transition from quiescence to the proliferative state. Decreased levels of p27 are found in various epithelial tumours originating from lung, breast, colon, ovary, oesophagus, thyroid and prostate. Defects in the gene encoding p27 (CDKN1B) are the cause of multiple endocrine neoplasia type 4 (MEN4) an inherited cancer of the thyroid.
Applications:
250 µg of p27 complex. Activated complex of three (3) full length, recombinant human proteins: p27, Cyclin E1, and CDK2. Phosphorylated in vitro using CDK2/Cyclin E1. For use in Enzyme Assays. Behaves as a substrate in ubiquitin assays.
Key Applications:
Enzyme Assay
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Species:
Human
Cascade Component:
Substrate
Brand Family:
Upstate
Specific Activity:
For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
Long Description:
Complex of N-terminal c-Myc-tagged, recombinant human p27 full length, 6His-tagged, recombinant human Cyclin E1 full length and untagged, recombinant human CDK2 full length, co-expressed by baculovirus in Sf21 insect cells. Purified using immobilized metal affinity chromatography. Phosphorylated in vitro using CDK2/Cyclin E1.
Product Name:
p27 complex, activated
Entrez Gene Number:
Alternate Names:
- p27kip1
- Cyclin-dependent kinase inhibitor 1B
- Kip1
Purification Method:
Purified using immobilized metal affinity chromatography.