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  • : 23-026M
  • : 250 µg
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Activity was measured by titrating 500ng of SCF βTrCP1 complex in a ubiquitination assay. Data shown for a representative lot.

Purity was assessed by SDS-PAGE and Coomassie blue staining using 3µg of active βTrCP1 complex. Confirmed identity as βTrCP1, Skp1, Cul1 and Rbx1 by m...

SCF-TrCP1 Protein Complex, Active, 250 μg

Description:
SCFβTrCP1 complex, active
Trade Name:
Upstate (Millipore)
Qty/Pk:
250 µg
Background Information:
The SCF (Skp1-Cul1-F-box protein) complexes represent the largest family of ubiquitin-protein ligases and mediate the ubiquitination of a broad spectrum of regulatory and signalling proteins in diverse cellular pathways. The SCF consists of three invariant components, Skp1, Cul1, and Rbx1 and an interchangeable subunit, an F-box protein which is responsible for recruiting specific substrates to be ubiquitinated by the SCF. SCFβTrCP1 mediates the ubiquitination and subsequent proteasomal degradation of a variety of proteins involved in various cellular processes, these include IκBα, an inhibitor of the transcription factor NFκB and β-catenin which is involved in the Wnt signalling pathway. A number of cancers, including those in breast, colon and pancreatic tissue have been associated with the deregulation of SCFβTrCP1
Applications:
250 µg of SCFβTrCP1 complex. Active complex of four (4) recombinant human enzymes: full length Skp1, full length Cul1, full length Rbx1, and βTrCP1 (amino acids 53-end). For use in Enzyme Assays. Functions as an E3 ligase in ubiquitination assays.
Key Applications:
Enzyme Assay
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Species:
Human
Cascade Component:
E3
Brand Family:
Upstate
Specific Activity:
For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
Long Description:
Complex of N-terminal GST-tagged, recombinant human βTrCP1 amino acids 53-end, N-terminal GST-tagged, recombinant human Skp1 full length, N-terminal 6His-tagged, recombinant human Cul1 full length and untagged recombinant human Rbx1 full length, co-expressed by baculovirus in Sf21 insect cells. Purified using glutathione sepharose.
Product Name:
SCFβTrCP1 complex, active
Entrez Gene Number:
Alternate Names:
  • SCF-β-TrCP
  • SCFFWD1
  • SCF-bTrCP1
Purification Method:
Purified using glutathione sepharose.