Ordering Information
- : 23-027M
- : 250 µg
Product Images
Activity was measured by titrating 364ng of β-catenin protein in a ubiquitination assay. Data shown for a representative lot.
Purity was assessed by SDS-PAGE and Coomassie blue staining using 3µg of activated β-catenin. Confirmed identity as β-catenin by mass spectrometry.
b-catenin Protein, activated, 250 μg
Description:
b-catenin, activated
Trade Name:
Upstate (Millipore)
Qty/Pk:
250 µg
Background Information:
β-catenin is a multifunctional protein; it is a central component of the cadherin cell adhesion complex, regulating the control of cell adhesion and plays an essential role in the Wingless/Wnt signaling pathway that governs cell fate in early embryogenesis. The regulation of β-catenin turnover in the cells is governed by its phosphorylation status which allows the targeting of ubiquitin-dependent proteolysis. Altered levels of protein expression as well as genetic mutations of β-catenin have been implicated in a number of human cancers.
Applications:
250 µg of β-catenin. Activated, full length, recombinant human β-catenin. N-terminal c-Myc, 6His-tagged. Phosphorylated in vitro using GSK-3a (Item # 14-492). For use in Enzyme Assays. Behaves as a substrate in ubiquitin assays.
Key Applications:
Enzyme Assay
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Species:
Human
Cascade Component:
Substrate
Brand Family:
Upstate
Specific Activity:
For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
Long Description:
N-terminal c-Myc, 6His-tagged, recombinant human β-catenin full length, expressed by baculovirus in Sf21 insect cells. Purified using immobilized metal affinity chromatography. Phosphorylated in vitro using GSK-3a (Item # 14-492).
Product Name:
b-catenin, activated
Entrez Gene Number:
Alternate Names:
Catenin beta-1
Purification Method:
Purified using immobilized metal affinity chromatography.